Anticorps Recombinant de lapin anti-MOSC2

MOSC2 Recombinant Antibody for WB, IHC, FC (Intra), ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, FC (Intra), ELISA

Conjugaison

Non conjugué

CloneNo.

240747G6

N° de cat : 83705-2-RR

Synonymes

Molybdenum cofactor sulfurase C-terminal domain-containing protein 2, Moco sulfurase C-terminal domain-containing protein 2, Mitochondrial amidoxime reducing component 2, mARC2, EC:1.7.-.-

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Galerie de données de validation

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  WB analysis using 83705-2-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83705-2-RR (MOSC2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis using 83705-2-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83705-2-RR (MOSC2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of human stomach cancer using 83705-2-RR

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 83705-2-RR (MOSC2 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach cancer using 83705-2-RR

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 83705-2-RR (MOSC2 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  FC experiment of HeLa using 83705-2-RR

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug MOSC2 Recombinant antibody (83705-2-RR, Clone:240747G6) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  Affinity and Kinetic Characterization of 83705-2-RR

  Biolayer interferometry (BLl) kinetic assays of 83705-2-RR against Human MOSC2 were performed. The affinity constant is 0.473 nM.

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Applications testées

• Résultats positifs en WB: tissu hépatique de souris, cellules HEK-293, cellules HUVEC, tissu hépatique de rat, tissu rénal de souris
• Résultats positifs en IHC: tissu de cancer de l'estomac humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en FC (Intra): cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochimie (IHC): IHC : 1:200-1:800
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

83705-2-RR cible MOSC2 dans les applications de WB, IHC, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: MOSC2 Protéine recombinante Ag20694
• Nom complet: MOCO sulphurase C-terminal domain containing 2
• Masse moléculaire calculée: 335 aa, 38 kDa
• Poids moléculaire observé: 35-38 kDa
• Numéro d’acquisition GenBank: BC011973
• Symbole du gène: MOSC2
• Identification du gène (NCBI): 54996
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

MOSC domain-containing protein 2 (also known as MOSC2), also known as MARC2, is a component of prodrug-converting system, reduces a multitude of N-hydroxylated prodrugs particularly amidoximes, leading to increased drug bioavailability. Also, MOSC2 may be involved in mitochondrial N(omega)-hydroxy-L-arginine (NOHA) reduction, regulating endogenous nitric oxide levels and biosynthesis. The reductase activity is regulated under adipogenic conditions, and down-regulation of the terminal component MOSC2 resulted in decreased lipid synthesis, suggesting a possible physiological role of this enzyme system and its component MOSC2 in lipogenesis(PMID: 22203676).

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