Anticorps Polyclonal de lapin anti-MPG

MPG Polyclonal Antibody for WB, IP, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, ELISA

Conjugaison

Non conjugué

Publications(3)

N° de cat : 11481-2-AP

Synonymes

3 alkyladenine DNA glycosylase, AAG, ADPG, anpg, APNG, CRA36.1, MDG, Mid1, MPG, N methylpurine DNA glycosylase, PIG11, PIG16

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Galerie de données de validation

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  WB analysis of HepG2 using 11481-2-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 11481-2-AP (MPG antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 11481-2-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 11481-2-AP (MPG antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 11481-2-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 11481-2-AP (MPG antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 11481-2-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 11481-2-AP (MPG antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IP experiment of HepG2 using 11481-2-AP

  IP result of anti-MPG (IP:11481-2-AP, 4ug; Detection:11481-2-AP 1:300) with HepG2 cells lysate 2200 ug.

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  IHC staining of human prostate cancer using 11481-2-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 11481-2-AP (MPG antibody) at dilution of 1:50 (under 10x lens).

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Applications testées

• Résultats positifs en WB: cellules HepG2, cellules Jurkat, cellules MCF-7
• Résultats positifs en IP: cellules HepG2,
• Résultats positifs en IHC: tissu de cancer de la prostate humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 3 publications below

Informations sur le produit

11481-2-AP cible MPG dans les applications de WB, IP, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: MPG Protéine recombinante Ag2034
• Nom complet: N-methylpurine-DNA glycosylase
• Masse moléculaire calculée: 293 aa, 32 kDa
• Poids moléculaire observé: 33 kDa
• Numéro d’acquisition GenBank: BC014991
• Symbole du gène: MPG
• Identification du gène (NCBI): 4350
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

N-methylpurine DNA glycosylase (MPG), an initiator glycosylase of the base excision repair (BER) pathway, typically repairs alkyl-induced DNA adducts, many of which are associated with genomic instability and tumorigenic risk [PMID:22801474] MPG initiates BER by removing TMZ-induced N3-methyladenine and N7-methylguanine base lesions, leaving abasic sites (AP sites) in DNA for further processing by BER [PMID:21377995]. MPG involves in modulating the genotoxic threshold response induced by the Sn2 alkylating agent ethyl methanesulfonate (EMS) [PMID:21071726].

Publications

Species	Application	Title
human	WB	EMBO Rep; LncRNA GUARDIN suppresses cellular senescence through a LRP130-PGC1α-FOXO4-p21-dependent signaling axis.; Authors - Xuedan Sun
human	WB	J Oncol; A Novel Pseudogene Methylation Signature to Predict Temozolomide Outcome in Non-G-CIMP Glioblastomas.; Authors - Bowen Li
mouse	WB	J Radiat Res; Abundance of BER-related proteins depends on cell proliferation status and the presence of DNA polymerase β.; Authors - Mizuki Yamamoto

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