Anticorps Polyclonal de lapin anti-MPHOSPH6

MPHOSPH6 Polyclonal Antibody for IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IP, IHC, ELISA

Conjugaison

Non conjugué

Publications

1

Avis (2)

N° de cat : 10695-1-AP

Synonymes

M phase phosphoprotein 6, MPHOSPH6, MPP, MPP 6, MPP6

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Galerie de données de validation

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  WB analysis of MCF-7 using 10695-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 10695-1-AP (MPHOSPH6 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 10695-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 10695-1-AP (MPHOSPH6 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of MDA-MB-453s using 10695-1-AP

  MDA-MB-453s cells were subjected to SDS PAGE followed by western blot with 10695-1-AP (MPHOSPH6 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 10695-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10695-1-AP (MPHOSPH6 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 10695-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10695-1-AP (MPHOSPH6 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 10695-1-AP

  IP result of anti-MPHOSPH6 (IP:10695-1-AP, 4ug; Detection:10695-1-AP 1:500) with HeLa cells lysate 3000 ug.

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  IHC staining of human breast cancer using 10695-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 10695-1-AP (MPHOSPH6 antibody) at dilution of 1:50 (under 10x lens).

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Applications testées

• Résultats positifs en WB: cellules MCF7, cellules HeLa, cellules MCF-7, cellules MDA-MB-453s
• Résultats positifs en IP: cellules HeLa,
• Résultats positifs en IHC: tissu de cancer du sein humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:200-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

10695-1-AP cible MPHOSPH6 dans les applications de WB, IP, IHC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: MPHOSPH6 Protéine recombinante Ag1080
• Nom complet: M-phase phosphoprotein 6
• Masse moléculaire calculée: 19 kDa
• Poids moléculaire observé: 19 kDa
• Numéro d’acquisition GenBank: BC005242
• Symbole du gène: MPHOSPH6
• Identification du gène (NCBI): 10200
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
		Cell Prolif; The 5.8S pre-rRNA maturation factor, M-phase phosphoprotein 6, is a female fertility factor required for oocyte quality and meiosis.; Authors - Rui-Rui Peng

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Tsimafei (Verified Customer) (12-17-2023)

Nice antibody that specifically recognise Mphosph6

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: mESCs

MPHOSPH6 Antibody Western Blot validation (1:1000 dilution) in mESCs (Cat no:10695-1-AP)

FH

Ralf (Verified Customer) (08-04-2022)

10µg of the total mouse protein were loaded on the gel. Antibodies also stain the ladder a bit.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000

MPHOSPH6 Antibody Western Blot validation (1:1000 dilution) in (Cat no:10695-1-AP)