Anticorps Recombinant de lapin anti-MPHOSPH9

MPHOSPH9 Recombinant Antibody for WB, FC (Intra), ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, FC (Intra), ELISA

Conjugaison

Non conjugué

CloneNo.

240182D6

N° de cat : 83397-5-RR

Synonymes

MPP9, MPP 9, M-phase phosphoprotein 9, 240182D6

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Galerie de données de validation

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  WB analysis using 83397-5-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83397-5-RR (MPHOSPH9 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis using 83397-5-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83397-5-RR (MPHOSPH9 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  FC experiment of A431 using 83397-5-RR

  1x10^6 A431 cells were intracellularly stained with 0.25 ug MPHOSPH9 Recombinant antibody (83397-5-RR, Clone:240182D6) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  Affinity and Kinetic Characterization of 83397-5-RR

  Biolayer interferometry (BLl) kinetic assays of 83397-5-RR against Human MPHOSPH9 were performed. The affinity constant is below 1 pM.

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules NIH/3T3, cellules PC-12, cellules SH-SY5Y, tissu cérébral de rat, tissu cérébral de souris
• Résultats positifs en FC (Intra): cellules A431,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

83397-5-RR cible MPHOSPH9 dans les applications de WB, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: MPHOSPH9 Protéine recombinante Ag22228
• Nom complet: M-phase phosphoprotein 9
• Masse moléculaire calculée: 1031 aa, 116 kDa
• Poids moléculaire observé: 116 kDa
• Numéro d’acquisition GenBank: BC130440
• Symbole du gène: MPHOSPH9
• Identification du gène (NCBI): 10198
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

MPHOSPH9, also known as MPP9, is a protein phosphorylated during mitosis. MPHOSPH9 negatively regulates cilia formation by recruiting the CP110-CEP97 complex at the distal end of the mother centriole in ciliary cells. MPHOSPH9 is a centrosome component that localizes to the distal and proximal ends of two centrioles(PMID: 30375385).

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