• Phare
  • Validé par KD/KO

Anticorps Monoclonal anti-MPO

MPO Monoclonal Antibody for FC (Intra), IF

Hôte / Isotype

Mouse / IgA

Réactivité testée

Humain, rat

Applications

IF, FC (Intra)

Conjugaison

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

4C11F6

N° de cat : CL488-66177

Synonymes

MPO, myeloperoxidase



Applications testées

Résultats positifs en IFtissu d'amygdalite humain,
Résultats positifs en cytométriecellules HL-60

Dilution recommandée

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

CL488-66177 cible MPO dans les applications de IF, FC (Intra) et montre une réactivité avec des échantillons Humain, rat

Réactivité Humain, rat
Hôte / Isotype Mouse / IgA
Clonalité Monoclonal
Type Anticorps
Immunogène MPO Protéine recombinante Ag17564
Nom complet myeloperoxidase
Masse moléculaire calculée 745 aa, 84 kDa
Poids moléculaire observé 90 kDa
Numéro d’acquisition GenBankBC130476
Symbole du gène MPO
Identification du gène (NCBI) 4353
Conjugaison CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Forme Liquide
Méthode de purification Chromatographie d'affinité thiophilique
Tampon de stockage PBS avec glycérol à 50 %, Proclin300 à 0,05 % et BSA à 0,5 %, pH 7,3.
Conditions de stockageStocker à -20 °C. Éviter toute exposition à la lumière. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The MPO gene encodes myeloperoxidase, a lysosomal hemoprotein located in the azurophilic granules of polymorphonuclear (PMN) leukocytes and monocytes. In response to stimulation, MPO is activated into a transient intermediate with potent antimicrobial oxidizing abilities(PMID:17650507). The mRNA is translated into a single protein of 90 kDa, which displays enzymatic activity and undergoes proteolytic maturation into a heavy chain of 59 kDa and a light chain of 13.5 kDa; these subunits then dimerize into the mature tetramer and the mature MPO is a heterotetramer composed of two identical heavy chains and two identical light chains(PMID:12773517). The 24-kDa material had a map identical to that of 13.5 kDa subunit and represents a dimer of the 13.5 kDa subunit (PMID:3008892). Defects in MPO are the cause of myeloperoxidase deficiency (MPOD). It has 3 isoforms produced by alternative splicing.

Protocole

Product Specific Protocols
IF protocol for CL Plus 488 MPO antibody CL488-66177Download protocol
FC protocol for CL Plus 488 MPO antibody CL488-66177Download protocol
Standard Protocols
Click here to view our Standard Protocols

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Sonja (Verified Customer) (08-04-2023)

The antibody works absolutely perfect in human FFPE tissue. The fluorescence intensity was very strong.

  • Applications: Immunohistochemistry, Immunofluorescence
  • Primary Antibody Dilution: 1:100
  • Cell Tissue Type: FFPE Human Tonsil
CL Plus 488 MPO Antibody Immunohistochemistry,Immunofluorescence validation (1:100 dilution) in FFPE Human Tonsil (Cat no:CL488-66177)