Anticorps Polyclonal de lapin anti-MPV17

• Phare
• Validé par KD/KO

MPV17 Polyclonal Antibody for IF, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IF, ELISA

Conjugaison

Non conjugué

Publications

11

N° de cat : 10310-1-AP

Synonymes

MPV17, Protein Mpv17, SYM1

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Galerie de données de validation

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  WB analysis using 10310-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10310-1-AP (MPV17 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 10310-1-AP

  WB result of MPV17 antibody (10310-1-AP; 1:1200; incubated at room temperature for 1.5 hours) with sh-Control and sh-MPV17 transfected HeLa cells.

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  IF Staining of HepG2 using 10310-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 10310-1-AP (MPV17 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules A549, cellules HeLa, cellules HepG2, tissu cardiaque de rat, tissu cardiaque de souris
• Résultats positifs en IF: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 4 publications below
• WB: See 10 publications below
• IF: See 3 publications below

Informations sur le produit

10310-1-AP cible MPV17 dans les applications de WB, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: MPV17 Protéine recombinante Ag0312
• Nom complet: MpV17 mitochondrial inner membrane protein
• Masse moléculaire calculée: 20 kDa
• Poids moléculaire observé: 20 kDa
• Numéro d’acquisition GenBank: BC001115
• Symbole du gène: MPV17
• Identification du gène (NCBI): 4358
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

MPV17, also termed as SYM1, encodes an inner mitochondrial membrane protein. It's potential candidate that may account for the mitochondrial (mt) DNA depletion syndromes (MDDS) characterized by a severe, tissue-specific decrease of mtDNA copy number and ultimate organ failure. It's a human ortholog of the mouse kidney disease gene Mpv17. Absence or malfunction of MPV17 causes oxidative phosphorylation (OXPHOS) failure and mtDNA depletion, not only in affected individuals but also in Mpv17-/- mice. MPV17 contribute to ROS homeostasis, but the downstream signaling is unknown yet. Catalog# 10310-1-AP is a rabbit polyclonal antibody raised against the full-length of human MPV17.

Publications

Species	Application	Title
human	WB,IF	Nat Genet; MPV17 encodes an inner mitochondrial membrane protein and is mutated in infantile hepatic mitochondrial DNA depletion.; Authors - Spinazzola Antonella A ; KO Validated
mouse	WB	Nucleic Acids Res; Aberrant ribonucleotide incorporation and multiple deletions in mitochondrial DNA of the murine MPV17 disease model.; Authors - Chloe F Moss
human	WB	Nucleic Acids Res; MPV17L2 is required for ribosome assembly in mitochondria.; Authors - Ilaria Dalla Rosa
mouse	WB	Mol Ther; AAV-mediated liver-specific MPV17 expression restores mtDNA levels and prevents diet-induced liver failure.; Authors - Emanuela Bottani ; KO Validated
mouse	WB	PLoS Genet; MPV17 Loss Causes Deoxynucleotide Insufficiency and Slow DNA Replication in Mitochondria.; Authors - Ilaria Dalla Rosa ; KO Validated
mouse	WB,IF	Clin Sci (Lond); Mitochondrial protein MPV17 promotes β-cell apoptosis in diabetogenesis; Authors - Qiaoli Tang

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