Anticorps Polyclonal de lapin anti-MR1

MR1 Polyclonal Antibody for WB, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications(7)

Avis (1)

N° de cat : 13260-1-AP

Synonymes

MHC class I-related gene protein, Major histocompatibility complex class I-related gene protein, HLALS, Class I histocompatibility antigen-like protein

---

Galerie de données de validation

• 

  WB analysis of THP-1 cells using 13260-1-AP

  WB result of MR1 antibody (13260-1-AP, 1:250) with IP samples of THP.1-MR1 cells, by Claudia Gonzalez-Lopez, MRC Human Immunology Unit, University of Oxford. (Green MR1 protein).

• 

  IF Staining of THP-1 cells using 13260-1-AP

  IF result of MR1 antibody (13260-1-AP, 1:50) with THP.1-MR1 cells, by Claudia Gonzalez-Lopez, MRC Human Immunology Unit, University of Oxford.

• 

  IF Staining of THP-1 using 13260-1-AP

  Immunofluorescent analysis of (4% PFA) fixed THP-1 cells using MR1 antibody (13260-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

---

Applications testées

• Résultats positifs en WB: cellules THP-1
• Résultats positifs en IF/ICC: cellules THP-1,

Dilution recommandée

• Western Blot (WB): WB : 1:100-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 3 publications below
• IF: See 3 publications below
• FC: See 1 publications below

Informations sur le produit

13260-1-AP cible MR1 dans les applications de WB, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: MR1 Protéine recombinante Ag3849
• Nom complet: major histocompatibility complex, class I-related
• Masse moléculaire calculée: 341 aa, 39 kDa
• Numéro d’acquisition GenBank: BC012485
• Symbole du gène: MR1
• Identification du gène (NCBI): 3140
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
human	WB,IF	Proc Natl Acad Sci U S A; Ligand-dependent downregulation of MR1 cell surface expression.; Authors - Mariolina Salio
mouse	IF	Carbohydr Polym; Lepidium meyenii Walpers polysaccharide and its cationic derivative re-educate tumor-associated macrophages for synergistic tumor immunotherapy.; Authors - Tingting Guo
human	WB	J Biol Chem; The P5-type ATPase ATP13A1 modulates major histocompatibility complex I-related protein 1 (MR1)-mediated antigen presentation.; Authors - Corinna A Kulicke
human	FC	Am J Pathol; Bile from Patients with Primary Sclerosing Cholangitis Contains Mucosal-Associated Invariant T-Cell Antigens.; Authors - Laura Valestrand
mouse	IF	Pigment Cell Melanoma Res; Topical antibiotics limit depigmentation in a mouse model of vitiligo; Authors - Ahmed Ahmed Touni
human	WB	Cancer Immunol Res; MR1 gene and protein expression are enhanced by inhibition of the extracellular signal-regulated kinase ERK; Authors - Daniel Constantin

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

---

FH

Tina (Verified Customer) (08-31-2020)

- PAGE was loaded with 80ug total protein - PVDF membrane blocked with milk - first AB dilution 1:250 (stored at -20°C, I could reuse the solution multiple times) - secondary AB I used HRP goat anti-rabbit and a high sensitive ECl to develop the Blot.

• Applications: Western Blot
• Primary Antibody Dilution: 1:250
• Cell Tissue Type: cell lines K562, primary liver cells

{{ptg:RelatedPrimaryAntibodies}}