Anticorps Polyclonal de lapin anti-MRE11

MRE11 Polyclonal Antibody for IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, IF, ChIP, ELISA

Conjugaison

Non conjugué

Publications

7

N° de cat : 10744-1-AP

Synonymes

ATLD, HNGS1, MRE11, MRE11 homolog 1, MRE11 homolog A, MRE11A, MRE11B

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Galerie de données de validation

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  WB analysis using 10744-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10744-1-AP (MRE11 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 10744-1-AP

  IP Result of anti-MRE11A (IP:10744-1-AP, 4ug; Detection:10744-1-AP 1:600) with HeLa cells lysate 1200ug.

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  IHC staining of human lymphoma using 10744-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma using 10744-1-AP (MRE11 antibody) at dilution of 1:50 (under 10x lens).

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  IF Staining of A431 using 10744-1-AP

  Immunofluorescent analysis of A431 cells using 10744-1-AP (MRE11 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules HeLa, cellules HepG2
• Résultats positifs en IP: cellules HeLa
• Résultats positifs en IHC: tissu de lymphome humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 7 publications below
• IHC: See 1 publications below
• IF: See 1 publications below
• ChIP: See 1 publications below

Informations sur le produit

10744-1-AP cible MRE11 dans les applications de WB, IP, IHC, IF, ChIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: MRE11 Protéine recombinante Ag1105
• Nom complet: MRE11 meiotic recombination 11 homolog A (S. cerevisiae)
• Masse moléculaire calculée: 81 kDa
• Poids moléculaire observé: 81 kDa
• Numéro d’acquisition GenBank: BC005241
• Symbole du gène: MRE11A
• Identification du gène (NCBI): 4361
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Mre11 (meiotic recombination 11), also named as Mre11A, is a component of the MRN( MRE11/RAD50/NBS1) complex which is a versatile complex, playing a key role in the sensing, processing, and repair of DSBs. MRE11 possesses both endonuclease and 3'-5' exonuclease activity. It contains two DNA-binding domains (DBDs), enabling it to bind both single-stranded DNA (ssDNA) and double-stranded DNA (dsDNA). After DSBs, MRE11 is loaded onto DSBs sites and cleaves DNA by cooperating with CtIP (CtBP [C terminal binding protein] interacting protein) to initiate end resection. It's reported that MRE11 is over-expressed in breast cancers. (PMID:38128537; 22914783)

Publications

Species	Application	Title
human	WB	Sci Transl Med; Guanosine diphosphate-mannose suppresses homologous recombination repair and potentiates antitumor immunity in triple-negative breast cancer; Authors - Jia-Han Ding
human	WB	Nat Commun; Crosstalk between SUMOylation and ubiquitylation controls DNA end resection by maintaining MRE11 homeostasis on chromatin; Authors - Tao Zhang
human	WB,IF	Oncogene; TRIM24 is critical for the cellular response to DNA double-strand breaks through regulating the recruitment of MRN complex; Authors - Ya Wang
human	WB,IHC,ChIP	Cell Death Dis; Acquired temozolomide resistance in MGMTlow gliomas is associated with regulation of homologous recombination repair by ROCK2.; Authors - Xin Zhang
human	WB	Nat Commun; A multi-functional role for the MCM8/9 helicase complex in maintaining fork integrity during replication stress; Authors - Wezley C. Griffin
rat	WB	J Physiol; Chronic fetal hypoxia disrupts the peri-conceptual environment in next-generation adult female rats.; Authors - C E Aiken