Anticorps Monoclonal anti-MRPS25

• Phare
• Validé par KD/KO

MRPS25 Monoclonal Antibody for WB, ELISA

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain, rat, souris

Applications

WB, ELISA

Conjugaison

Non conjugué

Publications(1)

CloneNo.

3B10B6

N° de cat : 67903-1-Ig

Synonymes

DKFZp313H0817, FLJ00023, MRP S25, MRPS25, RPMS25, S25mt

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Galerie de données de validation

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  WB analysis using 67903-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67903-1-Ig (MRPS25 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

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  WB analysis using 67903-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67903-1-Ig (MRPS25 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 67903-1-Ig

  WB result of MRPS25 antibody (67903-1-Ig; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MRPS25 transfected HepG2 cells.

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules 4T1, cellules HepG2, cellules HSC-T6, cellules Jurkat, cellules K-562, cellules NIH/3T3, cellules PC-12

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below

Informations sur le produit

67903-1-Ig cible MRPS25 dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: MRPS25 Protéine recombinante Ag7846
• Nom complet: mitochondrial ribosomal protein S25
• Masse moléculaire calculée: 20 kDa
• Poids moléculaire observé: 20 kDa
• Numéro d’acquisition GenBank: BC003590
• Symbole du gène: MRPS25
• Identification du gène (NCBI): 64432
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Mitochondrial ribosomal protein S25 (MRPS25) belongs to the ribosomal protein S25/L51 family. It is a component of the mitochondrial ribosome small subunit (28S). The variant segregated with the disease and substitutes a highly conserved proline residue with leucine (p.P72L) that, based on the high-resolution structure of the 28S ribosome, is predicted to compromise inter-protein contacts and destabilize the small subunit.

Publications

Species	Application	Title
human	WB	Cell Rep; A microscopy-based screen identifies cellular kinases modulating mitochondrial translation; Authors - Roya Yousefi

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