Anticorps Polyclonal de lapin anti-MRPS27

• Phare
• Validé par KD/KO

MRPS27 Polyclonal Antibody for IF, IHC, IP, WB,ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

26

Avis (1)

N° de cat : 17280-1-AP

Synonymes

KIAA0264, MRP S27, MRPS27, S27mt

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Galerie de données de validation

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  WB analysis of A431 using 17280-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 17280-1-AP (MRPS27 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human skeletal muscle using 17280-1-AP

  human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 17280-1-AP (MRPS27 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human heart using 17280-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 17280-1-AP (MRPS27 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A431 using 17280-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 17280-1-AP (MRPS27 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of A431 using 17280-1-AP

  IP Result of anti-MRPS27 (IP:17280-1-AP, 3ug; Detection:17280-1-AP 1:1000) with A431 cells lysate 1520ug.

• 

  IHC staining of human kidney using 17280-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 17280-1-AP (MRPS27 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human testis using 17280-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 17280-1-AP (MRPS27 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human testis using 17280-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 17280-1-AP (MRPS27 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human spleen using 17280-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 17280-1-AP (MRPS27 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human spleen using 17280-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 17280-1-AP (MRPS27 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human lung using 17280-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 17280-1-AP (MRPS27 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human lung using 17280-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 17280-1-AP (MRPS27 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human kidney using 17280-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 17280-1-AP (MRPS27 antibody) at dilution of 1:100 (under 10x lens).

• 

  IF Staining of HepG2 using 17280-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using MRPS27 rabbit poly (17280-1-AP, Green) and KRT18 mouse mAb (66187-1-Ig, Red) at dilution of 1:50, further stained with Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) for 17280-1-AP, and Alexa Fluor 594-conjugated AffiniPure Goat Anti-Mouse IgG (H+L) for 66187-1-Ig.

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Applications testées

• Résultats positifs en WB: cellules A431, tissu cardiaque humain, tissu de muscle squelettique humain
• Résultats positifs en IP: cellules A431
• Résultats positifs en IHC: tissu rénal humain, tissu pulmonaire humain, tissu splénique humain, tissu testiculaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 23 publications below
• IF: See 3 publications below

Informations sur le produit

17280-1-AP cible MRPS27 dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: MRPS27 Protéine recombinante Ag11047
• Nom complet: mitochondrial ribosomal protein S27
• Masse moléculaire calculée: 168 aa, 19 kDa, 48 kDa
• Poids moléculaire observé: 48 kDa
• Numéro d’acquisition GenBank: BC030521
• Symbole du gène: MRPS27
• Identification du gène (NCBI): 23107
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Genes encoding PPR (pentatricopeptide repeat)-containing proteins constitute one of the largest gene families in plants. The majority of these proteins are predicted to target organelles and to bind to RNA. Strikingly, there is a dearth of these proteins in mammals, although genomic searches reveal six candidates, all of which are also predicted to target the mitochondrion. One of these proteins, MRPS27is participated in transcription and translation respectively. [PMID:18939947] MRPS27is a component of the small subunit of the mitochondrial ribosome that is encoded by the nuclear genome [PMID: 11279123].

Publications

Species	Application	Title
human	WB	Cell Metab; The mitochondrial RNA-binding protein GRSF1 localizes to RNA granules and is required for posttranscriptional mitochondrial gene expression.; Authors - Antonicka Hana H
human	WB	Nat Commun; RNA modification landscape of the human mitochondrial tRNALys regulates protein synthesis.; Authors - Uwe Richter
mouse	WB	J Clin Invest; AFG3L2 supports mitochondrial protein synthesis and Purkinje cell survival.; Authors - Almajan Eva R ER
human	WB	Nucleic Acids Res; The human Obg protein GTPBP10 is involved in mitoribosomal biogenesis.; Authors - Elena Lavdovskaia
mouse	WB	J Cell Biol; Quality control of mitochondrial protein synthesis is required for membrane integrity and cell fitness.; Authors - Uwe Richter
human	WB	Proc Natl Acad Sci U S A; Mitochondrial ribosomal protein L12 selectively associates with human mitochondrial RNA polymerase to activate transcription.; Authors - Surovtseva Yulia V YV

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Xhuljana (Verified Customer) (03-01-2024)

WB in ripa lysate C2C12 cells

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: C2C12 cells

MRPS27 Antibody Western Blot validation (1:1000 dilution) in C2C12 cells (Cat no:17280-1-AP)