Anticorps Polyclonal de lapin anti-CD20

CD20 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugaison

Non conjugué

Publications

4

Avis (1)

N° de cat : 24828-1-AP

Synonymes

B lymphocyte antigen CD20, B1, Bp35, CD20, LEU 16, MS4A1, MS4A2, S7

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Galerie de données de validation

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  WB analysis using 24828-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 24828-1-AP (CD20 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of Raji using 24828-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 24828-1-AP (MS4A1,CD20 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of Raji using 24828-1-AP

  IP Result of anti-CD20 (IP:24828-1-AP, 4ug; Detection:24828-1-AP 1:600) with Raji cells lysate 1440ug.

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  IHC staining of human tonsillitis using 24828-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 24828-1-AP (CD20 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 24828-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 24828-1-AP (CD20 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 24828-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 24828-1-AP (CD20 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 24828-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 24828-1-AP (CD20 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 24828-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 24828-1-AP (CD20 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 24828-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 24828-1-AP (CD20 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 24828-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 24828-1-AP (CD20 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 24828-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 24828-1-AP (CD20 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lymphoma using 24828-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma slide using 24828-1-AP (MS4A1,CD20 Antibody) at dilution of 1:50.

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  IHC staining of human lymphoma using 24828-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma slide using 24828-1-AP (MS4A1,CD20 Antibody) at dilution of 1:50.

• 

  IHC staining of human tonsillitis using 24828-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis slide using 24828-1-AP (MS4A1,CD20 Antibody) at dilution of 1:50.

• 

  IHC staining of human tonsillitis using 24828-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis slide using 24828-1-AP (MS4A1,CD20 Antibody) at dilution of 1:50.

• 

  IF Staining of human tonsillitis using 24828-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CD20 antibody (24828-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CD3 antibody (60181-1-Ig, Clone: 3F3A1, red).

• 

  IF Staining of human tonsillitis using 24828-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CD20 antibody (24828-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CD3 antibody (60181-1-Ig, Clone: 3F3A1, red).

• 

  IF Staining of Raji using 24828-1-AP

  Immunofluorescent analysis of (4% PFA) fixed Raji cells using CD20 antibody (24828-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of human tonsillitis using 24828-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CD20 antibody (24828-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CD3 antibody (60181-1-Ig, Clone: 3F3A1, red).

• 

  IF Staining of human tonsillitis using 24828-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using 24828-1-AP (CD20 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of human tonsillitis using 24828-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using 24828-1-AP (CD20 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of human tonsillitis using 24828-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using 24828-1-AP (CD20 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of human tonsillitis using 24828-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using PD-1/CD279 mouse mAb (66220-1-Ig) at dilution of 1:50 and CD20 rabbit pAb (24828-1-AP) at dilution of 1:50, further stained with Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L) for 66220-1-Ig, and Alexa Fluor 594-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) for 24828-1-AP.

• 

  IF Staining of human tonsillitis using 24828-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using PD-1/CD279 mouse mAb (66220-1-Ig) at dilution of 1:50 and CD20 rabbit pAb (24828-1-AP) at dilution of 1:50, further stained with Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L) for 66220-1-Ig, and Alexa Fluor 594-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) for 24828-1-AP.

• 

  FC experiment of Raji using 24828-1-AP

  1X10^6 Raji cells were stained with 0.2ug MS4A1,CD20 antibody (24828-1-AP, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-Goat anti-Rabbit IgG with dilution 1:100.

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Applications testées

• Résultats positifs en WB: cellules Daudi, cellules Raji, cellules Ramos
• Résultats positifs en IP: cellules Raji
• Résultats positifs en IHC: tissu d'amygdalite humain, tissu de cancer du côlon humain, tissu de cancer du sein humain, tissu de lymphome humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: tissu d'amygdalite humain, cellules Raji
• Résultats positifs en cytométrie: cellules Raji

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:8000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF): IF : 1:50-1:500
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below
• IHC: See 3 publications below

Informations sur le produit

24828-1-AP cible CD20 dans les applications de WB, IP, IHC, IF, FC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CD20 Protéine recombinante Ag17309
• Nom complet: membrane-spanning 4-domains, subfamily A, member 1
• Masse moléculaire calculée: 297 aa, 33 kDa
• Poids moléculaire observé: 35 kDa
• Numéro d’acquisition GenBank: BC002807
• Symbole du gène: MS4A1
• Identification du gène (NCBI): 931
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CD20 is a 33-37 kDa transmembrane phosphoprotein. CD20 is a B-lymphocyte surface molecule that is widely expressed during B-cell ontogeny, from early pre-B-cell developmental stages until final differentiation into plasma cells. CD20 functions as calcium-permeable cation channel. It is involved in the regulation of B-cell activation and proliferation. CD20 serves as a useful target for antibody-mediated therapeutic depletion of B-cells.

Publications

Species	Application	Title
human	IHC	J Immunother Cancer; Infiltrating pattern and prognostic value of tertiary lymphoid structures in resected non-functional pancreatic neuroendocrine tumors.; Authors - Wu-Hu Zhang
human	IHC	Clin Exp Immunol; CMTM6: increased circulating level and up-regulated expression in labial salivary glands in patients with primary Sjogren's syndrome.; Authors - Sirui Qian
human	WB	Acta Physiol (Oxf); Acute physical exercise and long-term individual shear rate therapy increase telomerase activity in human peripheral blood mononuclear cells.; Authors - A Zietzer
human	IHC	Int J Mol Sci; CXCR4 Expressed by Tumor-Infiltrating B Cells in Gastric Cancer Related to Survival in the Tumor Microenvironment: An Analysis Combining Single-Cell RNA Sequencing with Bulk RNA Sequencing; Authors - Chen Su

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Emma (Verified Customer) (11-29-2021)

Works well by IF on FFPE tissue. Antigen retrieval Tris-EDTA pH 9. Also works by IHC with sodium citrate antigen retrieval.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:50
• Cell Tissue Type: Prostate

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