Anticorps Polyclonal de lapin anti-MSH2

• Phare
• Validé par KD/KO

MSH2 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (1)

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugaison

Non conjugué

Publications

24

N° de cat : 15520-1-AP

Synonymes

COCA1, FCC1, hMSH2, HNPCC, HNPCC1, LCFS2, MSH2, MutS protein homolog 2

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Galerie de données de validation

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  WB analysis using 15520-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15520-1-AP (MSH2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 15520-1-AP

  WB results of MSH2 (15520-1-AP, 1:5000) antibody with si-Control and si-MSH2 transfected HeLa cells..

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  WB analysis using 15520-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15520-1-AP (MSH2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 15520-1-AP

  IP Result of anti-MSH2 (IP:15520-1-AP, 5ug; Detection:15520-1-AP 1:500) with HeLa cells lysate 2000ug.

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  IHC staining of human stomach cancer using 15520-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 15520-1-AP (MSH2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach cancer using 15520-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 15520-1-AP (MSH2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsil using 15520-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsil using 15520-1-AP (MSH2 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human tonsil using 15520-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsil using 15520-1-AP (MSH2 antibody) at dilution of 1:100 (under 40x lens).

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  IF Staining of HeLa using 15520-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using MSH2 antibody (15520-1-AP) at dilution of 1:100 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  FC experiment of HeLa using 15520-1-AP

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human MSH2 (15520-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules Caco-2, cellules SW480
• Résultats positifs en IP: cellules HeLa
• Résultats positifs en IHC: tissu de cancer de l'estomac humain, tissu amygdalien humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HeLa,
• Résultats positifs en cytométrie: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500
• Flow Cytometry (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 4 publications below
• WB: See 14 publications below
• IHC: See 11 publications below
• IF: See 3 publications below
• IP: See 1 publications below

Informations sur le produit

15520-1-AP cible MSH2 dans les applications de WB, IP, IHC, IF, FC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: MSH2 Protéine recombinante Ag7835
• Nom complet: mutS homolog 2, colon cancer, nonpolyposis type 1 (E. coli)
• Masse moléculaire calculée: 105 kDa
• Poids moléculaire observé: 100 kDa-105 kDa
• Numéro d’acquisition GenBank: BC021566
• Symbole du gène: MSH2
• Identification du gène (NCBI): 4436
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

MSH2, named for its homologous to the E.coli MutS gene, takes part in DNA mismatch repair(MMR) to maintain the stability of DNA. MSH2 contains a helix-turn-helix domain, which responds for binding to DNA. Also, MSH2 has a direct role in mutation avoidance and micro-satellite stability in cell.

Publications

Species	Application	Title
human	WB	Leukemia; Targeting DNA polymerase β elicits synthetic lethality with mismatch repair deficiency in acute lymphoblastic leukemia; Authors - Ji-Yuan Teng ; KO Validated
human	WB	J Immunother Cancer; KALRN mutations promote antitumor immunity and immunotherapy response in cancer.; Authors - Mengyuan Li
human	WB, IHC	Hum Mol Genet; Lack of association of somatic CAG repeat expansion with striatal neurodegeneration in HD knock-in animal models.; Authors - Dazhang Bai ; KD Validated
human	WB	Cancer Prev Res (Phila); Estrogen stimulates the expression of mismatch repair gene hMLH1 in colonic epithelial cells.; Authors - Jin Peng P
human	IHC	Front Oncol; Molecular Classification in Patients With Endometrial Cancer After Fertility-Preserving Treatment: Application of ProMisE Classifier and Combination of Prognostic Evidence.; Authors - Xuting Ran
human	WB	J Biol Chem; Ectopic expression of human MutS homologue 2 on renal carcinoma cells is induced by oxidative stress with interleukin-18 promotion via p38 mitogen-activated protein kinase (MAPK) and c-Jun N-terminal kinase (JNK) signaling pathways.; Authors - Mo Chen C