Anticorps Polyclonal de lapin anti-MSH6

• Phare
• Validé par KD/KO

MSH6 Polyclonal Antibody for FC, IF, IHC, IP, WB,ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugaison

Non conjugué

Publications

7

N° de cat : 18120-1-AP

Synonymes

G/T mismatch binding protein, GTBP, GTMBP, hMSH6, HNPCC5, HSAP, MSH6, MutS alpha 160 kDa subunit, mutS homolog 6 (E. coli), p160

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Galerie de données de validation

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  WB analysis of HEK-293 using 18120-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 18120-1-AP (MSH6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 18120-1-AP

  WB result of MSH6 antibody (18120-1-AP, 1:6000) with si-Control and si-MSH6 transfected HEK293 cells.

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  WB analysis of HEK-293 using 18120-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 18120-1-AP (MSH6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 18120-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 18120-1-AP (MSH6 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of COLO 320 using 18120-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 18120-1-AP (MSH6 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of HEK-293 using 18120-1-AP

  IP Result of anti-MSH6 (IP:18120-1-AP, 4ug; Detection:18120-1-AP 1:600) with HEK-293 cells lysate 1120ug.

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  IHC staining of human colon using 18120-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 18120-1-AP (MSH6 Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human colon using 18120-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 18120-1-AP (MSH6 Antibody) at dilution of 1:200 (under 10x lens).

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  IF Staining of HeLa using 18120-1-AP

  Immunofluorescent analysis of Hela cells, using MSH6 antibody 18120-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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  FC experiment of HEK-293 using 18120-1-AP

  1X10^6 HEK-293 cells were intracellularly stained with 0.2 ug Anti-Human MSH6 (18120-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (green), and 0.2 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with 0.1% TritonX-100.

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Applications testées

• Résultats positifs en WB: cellules HEK-293, HEK-293
• Résultats positifs en IP: cellules HEK-293
• Résultats positifs en IHC: tissu de côlon humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HeLa
• Résultats positifs en cytométrie: cellules HEK-293,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:10-1:100
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 5 publications below
• IHC: See 2 publications below
• IP: See 1 publications below

Informations sur le produit

18120-1-AP cible MSH6 dans les applications de WB, IP, IHC, IF, FC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: MSH6 Protéine recombinante Ag12645
• Nom complet: mutS homolog 6 (E. coli)
• Masse moléculaire calculée: 153 kDa
• Poids moléculaire observé: 150-160 kDa
• Numéro d’acquisition GenBank: BC004246
• Symbole du gène: MSH6
• Identification du gène (NCBI): 2956
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

MSH6, also named as DNA mismatch repair protein Msh6 or GTMBP, is a 1360 amino acid protein, which contains one PWWP domain and belongs to the DNA mismatch repair MutS family. MSH6 localizes in the nucleus and is a component of the post-replicative DNA mismatch repair system. Msh2 and Msh6 form a protein complex required to repair mismatches generated during DNA replication.

Publications

Species	Application	Title
human	WB	Mol Cancer; CircLIFR synergizes with MSH2 to attenuate chemoresistance via MutSα/ATM-p73 axis in bladder cancer.; Authors - Hui Zhang
human	WB	Oncotarget; Targeted silencing of SOX2 by an artificial transcription factor showed antitumor effect in lung and esophageal squamous cell carcinoma.; Authors - Etsuko Yokota
human	IHC	Front Oncol; Molecular Classification in Patients With Endometrial Cancer After Fertility-Preserving Treatment: Application of ProMisE Classifier and Combination of Prognostic Evidence.; Authors - Xuting Ran
human	WB	DNA Cell Biol; MSH6 Aggravates the Hypoxic Microenvironment via Regulating HIF1A to Promote the Metastasis of Glioblastoma Multiforme.; Authors - Ying Shi
mouse	WB	Nucleic Acids Res; Resolution of sequence divergence for repeat-mediated deletions shows a polarity that is mediated by MLH1; Authors - Hannah Trost
human	IHC	Front Oncol; The MSH2 c.793-1G>A variant disrupts normal splicing and is associated with Lynch syndrome; Authors - Yiming Li