Anticorps Polyclonal de lapin anti-GDF8/Myostatin

• Phare
• Validé par KD/KO

GDF8/Myostatin Polyclonal Antibody for IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (4)

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

30

N° de cat : 19142-1-AP

Synonymes

GDF 8, GDF8, GDF-8, GDF8/Myostatin, MSTN, myostatin

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Galerie de données de validation

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  WB analysis using 19142-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 19142-1-AP (GDF8/Myostatin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse heart using 19142-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 19142-1-AP (GDF8/Myostatin antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of PC-3 using 19142-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 19142-1-AP (GDF8/Myostatin antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 19142-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 19142-1-AP (GDF8/Myostatin antibody at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human heart using 19142-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 19142-1-AP (GDF8/Myostatin antibody at dilution of 1:200 (under 10x lens).

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  IHC staining of human heart using 19142-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 19142-1-AP (GDF8/Myostatin antibody at dilution of 1:200 (under 40x lens).

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  IHC staining of mouse brain using 19142-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 19142-1-AP (GDF8/Myostatin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: tissu placentaire humain, cellules PC-3, tissu cardiaque de souris, tissu hépatique de souris
• Résultats positifs en IHC: tissu cardiaque humain, tissu cérébral de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 26 publications below
• IHC: See 5 publications below
• IF: See 2 publications below

Informations sur le produit

19142-1-AP cible GDF8/Myostatin dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: rat, bovin, Humain, poisson-zèbre, porc, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: GDF8/Myostatin Protéine recombinante Ag13605
• Nom complet: myostatin
• Masse moléculaire calculée: 375 aa, 43 kDa
• Poids moléculaire observé: 50 and 35 kDa
• Numéro d’acquisition GenBank: BC074757
• Symbole du gène: MSTN
• Identification du gène (NCBI): 2660
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Myostatin, also known as GDF-8, is a member of the TGF-beta superfamily. Myostatin is expressed in the myotome and developing skeletal muscles. Myostatin negatively regulates skeletal muscle growth and development through the regulation of anabolic and catabolic pathways in skeletal muscles. Myostatin immunoreactive bands could be identified at ∼50, 32-35, and 16 kDa, which correspond to the precursor, glycosylated dimmer, and monomer, respectively (PMID: 17711997).

Publications

Species	Application	Title
mouse	WB	J Cachexia Sarcopenia Muscle; Potential therapeutic interventions for chronic kidney disease-associated sarcopenia via indoxyl sulfate-induced mitochondrial dysfunction.; Authors - Yuki Enoki
mouse	WB	Br J Pharmacol; Inhibition of heat shock protein (HSP) 90 reverses signal transducer and activator of transcription (STAT) 3-mediated muscle wasting in cancer cachexia mice.; Authors - Mengyuan Niu
human,rat	WB,IHC,IF	Int J Mol Sci; Renal Ischemia/Reperfusion Early Induces Myostatin and PCSK9 Expression in Rat Kidneys and HK-2 Cells.; Authors - Chiara Barisione ; KD Validated
mouse	WB	iScience; Phosphate depletion in insulin-insensitive skeletal muscle drives AMPD activation and sarcopenia in chronic kidney disease; Authors - Ana Andres-Hernando
mouse	WB	Aging (Albany NY); Vitellogenin 2 promotes muscle development and stimulates the browning of white fat; Authors - Yilei Li
mouse	WB	Sci Rep; Indoxyl sulfate potentiates skeletal muscle atrophy by inducing the oxidative stress-mediated expression of myostatin and atrogin-1.; Authors - Yuki Enoki