Anticorps Polyclonal de lapin anti-MTA2

• Phare
• Validé par KD/KO

MTA2 Polyclonal Antibody for IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, CoIP, ChIP, ELISA

Conjugaison

Non conjugué

Publications

4

N° de cat : 17554-1-AP

Synonymes

Metastasis associated 1 like 1, MTA1 L1 protein, MTA1L1, MTA2, PID

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Galerie de données de validation

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  WB analysis of HEK-293 using 17554-1-AP

  WB result of MTA2 antibody (17554-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MTA2 transfected HEK-293 cells.

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  WB analysis using 17554-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17554-1-AP (MTA2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse thymus using 17554-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 17554-1-AP (MTA2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 17554-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 17554-1-AP (MTA2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 17554-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 17554-1-AP (MTA2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse thymus using 17554-1-AP

  IP Result of anti-MTA2 (IP:17554-1-AP, 4ug; Detection:17554-1-AP 1:500) with mouse thymus tissue lysate 2800ug.

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  IHC staining of human colon using 17554-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon using 17554-1-AP (MTA2 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human colon using 17554-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon using 17554-1-AP (MTA2 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human heart using 17554-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 17554-1-AP (MTA2 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human heart using 17554-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 17554-1-AP (MTA2 antibody) at dilution of 1:100 (under 40x lens).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules HEK-293, cellules Jurkat, tissu de thymus de souris
• Résultats positifs en IP: tissu de thymus de souris
• Résultats positifs en IHC: tissu de côlon humain, tissu cardiaque humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 3 publications below
• CoIP: See 1 publications below
• ChIP: See 1 publications below

Informations sur le produit

17554-1-AP cible MTA2 dans les applications de WB, IP, IHC, CoIP, ChIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: MTA2 Protéine recombinante Ag11713
• Nom complet: metastasis associated 1 family, member 2
• Masse moléculaire calculée: 668 aa, 75 kDa
• Poids moléculaire observé: 70-75 kDa
• Numéro d’acquisition GenBank: BC053650
• Symbole du gène: MTA2
• Identification du gène (NCBI): 9219
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Metastasis-associated protein MTA2, also named as MTA1L1 or PID, is a 668 amino acid protein, which contains one BAH domain, one ELM2 domain, one GATA-type zinc finger and one SANT domain. MTA2 localizes in the nucleus and is widely expressed in many tissues. MTA2 may be involved in the regulation of gene expression as repressor and activator.

Publications

Species	Application	Title
human	WB	Nat Commun; Identification of proximal SUMO-dependent interactors using SUMO-ID.; Authors - Orhi Barroso-Gomila
human	WB	EMBO Rep; miR-AB, a miRNA-based shRNA viral toolkit for multicolor-barcoded multiplex RNAi at a single-cell level.; Authors - Dapeng Wang
human	WB	Cell Mol Gastroenterol Hepatol; Targeting HNRNPM Inhibits Cancer Stemness and Enhances Antitumor Immunity in Wnt-activated Hepatocellular Carcinoma.; Authors - Gui-Qi Zhu ; KD Validated
	CoIP,ChIP	Cancer Sci; MTA2 promotes the metastasis of esophageal squamous cell carcinoma via EIF4E-Twist feedback loop.; Authors - Su-Li Dai