Anticorps Polyclonal de lapin anti-MTAP

• Phare
• Validé par KD/KO

MTAP Polyclonal Antibody for IF, IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

25

Avis (2)

N° de cat : 11475-1-AP

Synonymes

c86fus, MSAP, MTA phosphorylase, MTAP, MTAPase

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Galerie de données de validation

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  WB analysis of HEK-293 using 11475-1-AP

  WB result of MTAP antibody (11475-1-AP; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MTAP transfected HEK-293 cells.

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  WB analysis using 11475-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11475-1-AP (MTAP antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  WB analysis using 11475-1-AP

  Various lysates from MTAP-expressing cells (Raji, NIH3T3, HeLa, and HEK293) and MTAP deficient cells (MCF7 and A549) were subjected to SDS PAGE followed by western blot with 11475-1-AP (MTAP antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse heart using 11475-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 11475-1-AP (MTAP Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 11475-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 11475-1-AP (MTAP Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human heart using 11475-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 11475-1-AP (MTAP antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon cancer using 11475-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 11475-1-AP (MTAP antibody) at dilution of 1:50 (under 10x lens).

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  IF Staining of NIH/3T3 using 11475-1-AP

  Immunofluorescent analysis of (4% PFA) fixed NIH/3T3 cells using 11475-1-AP (MTAP antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules C6, cellules HeLa, cellules HT-29, cellules NIH/3T3, cellules Raji, tissu cardiaque de souris, tissu cardiaque humain
• Résultats positifs en IHC: tissu de cancer du côlon humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules NIH/3T3

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:12000
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 11 publications below
• IHC: See 19 publications below

Informations sur le produit

11475-1-AP cible MTAP dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: MTAP Protéine recombinante Ag2051
• Nom complet: methylthioadenosine phosphorylase
• Masse moléculaire calculée: 283 aa, 31 kDa
• Poids moléculaire observé: 31 kDa
• Numéro d’acquisition GenBank: BC018625
• Symbole du gène: MTAP
• Identification du gène (NCBI): 4507
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

MTAP is a 5-deoxy-5-methylthioadenosine (MTA) phosphorylase, converting MTA to salvageable intermediates including adenine and 5-methylthioribose-1-phosphate. MTAP is abundant in normal cells, but it is frequently found to be deleted in a variety of cancers. Its deficiency is common in cancer cell lines as well as in primary leukemia, lung cancer, melanoma, bladder cancer, gliomas and breast cancer.

Publications

Species	Application	Title
human	WB	J Clin Invest; SHARPIN-mediated regulation of protein arginine methyltransferase 5 controls melanoma growth.; Authors - Hironari Tamiya
human	WB,IHC	Nat Commun; MTAP deficiency creates an exploitable target for antifolate therapy in 9p21-loss cancers.; Authors - Omar Alhalabi
human	IHC	Nat Commun; Pharmacological polyamine catabolism upregulation with methionine salvage pathway inhibition as an effective prostate cancer therapy.; Authors - Hayley C Affronti
human	IHC	Clin Cancer Res; Homozygous deletion of MTAP gene as a poor prognosticator in gastrointestinal stromal tumors.; Authors - Huang Hsuan-Ying HY
human	WB	Oncoimmunology; Suppressive effects of tumor cell-derived 5'-deoxy-5'-methylthioadenosine on human T cells.; Authors - Frederik C Henrich
human	IHC	J Pathol; Methylthioadenosine phosphorylase and activated insulin-like growth factor-1 receptor/insulin receptor: potential therapeutic targets in chordoma.; Authors - Sommer Josh J

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Satyendra (Verified Customer) (07-16-2021)

I have marked the knockout as KO

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HEK 293

MTAP Antibody Western Blot validation (1:1000 dilution) in HEK 293 (Cat no:11475-1-AP)

FH

Hua (Verified Customer) (10-21-2020)

Observed band at the right size with a clean background; strong signal.

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: mouse liver lysate

MTAP Antibody Western Blot validation (1:2000 dilution) in mouse liver lysate (Cat no:11475-1-AP)