Anticorps Recombinant de lapin anti-MTCO2

• Phare
• Validé par KD/KO

MTCO2 Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Conjugaison

Non conjugué

CloneNo.

230448C9

N° de cat : 83132-7-RR

Synonymes

COX2, 230448C9, COII, COX 2, COXII

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Galerie de données de validation

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  WB analysis of 143B.TK.P0 using 83132-7-RR

  WB result of MTCO2 antibody (MTCO2; 1:3000; incubated at room temperature for 1.5 hours) with 143B and 143B.TK.ρ0 cell. 143B is wild-type cell line, 143B.TK.ρ0 is mtDNA deletion cell line.

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  WB analysis using 83132-7-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83132-7-RR (MTCO2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 83132-7-RR

  IP result of anti-MTCO2 (IP:83132-7-RR, 4ug; Detection:83132-7-RR 1:3000) with HeLa cells lysate 1750 ug.

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  IHC staining of human stomach cancer using 83132-7-RR

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 83132-7-RR (MTCO2 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer(pH9).

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  IHC staining of human ovarian cancer using 83132-7-RR

  Immunohistochemical analysis of paraffin-embedded human ovarian cancer slide using 83132-7-RR (MTCO2 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 83132-7-RR

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using MTCO2 antibody (83132-7-RR, Clone: 230448C9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red).

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  FC experiment of HeLa using 83132-7-RR

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug Anti-Human MTCO2 (83132-7-RR, Clone:230448C9) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug rabbit IgG isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: cellules HepG2, cellules A549, cellules HeLa, cellules MDA-MB-231, cellules U-251
• Résultats positifs en IP: cellules HeLa,
• Résultats positifs en IHC: human ovarian cancer, tissu de cancer de l'estomac humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HepG2,
• Résultats positifs en FC (Intra): cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

83132-7-RR cible MTCO2 dans les applications de WB, IHC, IF/ICC, FC (Intra), IP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: cytochrome c oxidase II
• Masse moléculaire calculée: 26 kDa
• Poids moléculaire observé: 23-26 kDa
• Numéro d’acquisition GenBank: YP_003024029
• Symbole du gène: MTCO2
• Identification du gène (NCBI): 4513
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

MTCO2, also named as COII, COXII, and COX2, belongs to the cytochrome c oxidase subunit 2 family. It is the component of the respiratory chain that catalyzes the reduction of oxygen to water. Subunits 1-3 form the functional core of the enzyme complex. Subunit 2 transfers the electrons from cytochrome c via its binuclear copper A center to the bimetallic center of the catalytic subunit 1. Defects in COX2 are a cause of mitochondrial complex IV deficiency (MT-C4D).

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