Anticorps Polyclonal de lapin anti-AEG-1/MTDH-Specific

• Phare
• Validé par KD/KO

AEG-1/MTDH-Specific Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (1)

Applications

WB, RIP, IP, IHC, IF, CoIP, ELISA

Conjugaison

Non conjugué

Publications

51

Avis (1)

N° de cat : 13860-1-AP

Synonymes

3D3, 3D3/LYRIC, AEG 1, AEG1, AEG-1/MTDH-Specific, LYRIC, LYRIC/3D3, metadherin, Metastasis adhesion protein, MTDH, Protein LYRIC

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Galerie de données de validation

• 

  WB analysis of MCF-7 using 13860-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 13860-1-AP (AEG-1/MTDH-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 13860-1-AP

  WB result of AEG-1/MTDH-Specific antibody (13860-1-AP; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-AEG-1/MTDH-Specific transfected HeLa cells.

• 

  WB analysis of A549 cells using 13860-1-AP

  WB result of AEG-1/MTDH antibody (13860-1-AP, 1:2000) with si-Control and si-MTDH transfected A549 cells.

• 

  WB analysis of RAW 264.7 using 13860-1-AP

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 13860-1-AP (AEG-1/MTDH-Specific antibody at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse spleen using 13860-1-AP

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 13860-1-AP (AEG-1/MTDH-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 13860-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 13860-1-AP (AEG-1/MTDH-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 13860-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 13860-1-AP (AEG-1/MTDH-Specific antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 13860-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 13860-1-AP (AEG-1/MTDH-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 13860-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 13860-1-AP (AEG-1/MTDH-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of DU 145 using 13860-1-AP

  DU 145 cells were subjected to SDS PAGE followed by western blot with 13860-1-AP (AEG-1/MTDH-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A375 using 13860-1-AP

  A375 cells were subjected to SDS PAGE followed by western blot with 13860-1-AP (AEG-1/MTDH-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 13860-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 13860-1-AP (AEG-1/MTDH-Specific antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of MCF-7 using 13860-1-AP

  IP Result of anti-AEG-1/MTDH-Specific (IP:13860-1-AP, 3ug; Detection:13860-1-AP 1:1000) with MCF-7 cells lysate 3250ug.

• 

  IHC staining of human breast cancer using 13860-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13860-1-AP (AEG-1/MTDH-Specific antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 13860-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13860-1-AP (AEG-1/MTDH-Specific antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 13860-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 13860-1-AP (AEG-1/MTDH-Specific antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 13860-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 13860-1-AP (AEG-1/MTDH-Specific antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 13860-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13860-1-AP (AEG-1/MTDH-Specific antibody at dilution of 1:50 (under 10x lens).

• 

  IHC staining of mouse brain using 13860-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13860-1-AP (AEG-1/MTDH-Specific antibody at dilution of 1:50 (under 40x lens).

• 

  IF Staining of MCF-7 using 13860-1-AP

  Immunofluorescent analysis of MCF-7 cells, using MTDH antibody 13860-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

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Applications testées

• Résultats positifs en WB: cellules MCF-7, cellules A375, cellules A549, cellules DU 145, cellules HeLa, cellules HepG2, cellules HL-60, cellules PC-3, cellules RAW 264.7, tissu splénique de souris
• Résultats positifs en IP: cellules MCF-7
• Résultats positifs en IHC: tissu de cancer du sein humain, tissu cérébral de souris, tissu de cancer du foie humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules MCF-7

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:200-1:800
• Immunofluorescence (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 12 publications below
• WB: See 40 publications below
• IHC: See 24 publications below
• IF: See 5 publications below
• IP: See 2 publications below
• CoIP: See 1 publications below
• RIP: See 1 publications below

Informations sur le produit

13860-1-AP cible AEG-1/MTDH-Specific dans les applications de WB, RIP, IP, IHC, IF, CoIP, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: AEG-1/MTDH-Specific Protéine recombinante Ag4840
• Nom complet: metadherin
• Masse moléculaire calculée: 582 aa, 64 kDa
• Poids moléculaire observé: 65-70 kDa
• Numéro d’acquisition GenBank: BC045642
• Symbole du gène: MTDH
• Identification du gène (NCBI): 92140
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

MTDH, also known as AEG-1, is a single-pass transmembrane protein with its gene located at chromosome 8q22 , the abnormalities of which have been identified in several tumor types. MTDH is overexpressed in a number of malignancies, such as breast cancer, malignant glioma, and neuroblastoma , and its overexpression is associated with poor clinical outcome. MTDH plays an important role in the regulation of carcinogenesis and tumor progression. This antibody got two bands about 70 kDa and 80 kDa in western blotting, possibly due to posttranslational modification. This antibody is specific to AEG-1/MTDH.

Publications

Species	Application	Title
human	IF, WB, IP, IHC	J Crohns Colitis; MTDH promotes intestinal inflammation by positively regulating TLR signaling.; Authors - Lijuan Wang
human	WB	Oncogene; MicroRNA-375 targets AEG-1 in hepatocellular carcinoma and suppresses liver cancer cell growth in vitro and in vivo.; Authors - He X-X XX
		Mol Ther; Role of HCP5-miR-139-RUNX1 Feedback Loop in Regulating Malignant Behavior of Glioma Cells.; Authors - Hao Teng
human	WB	Mol Ther Nucleic Acids; PMID: 28624193; Authors - Yin-Ping Fan
human	WB,IHC	Oncotarget; MiR-497 suppresses angiogenesis and metastasis of hepatocellular carcinoma by inhibiting VEGFA and AEG-1.; Authors - Jing-Jun Yan
human	IHC	Int J Cancer; Increased expression of metadherin protein predicts worse disease-free and overall survival in laryngeal squamous cell carcinoma.; Authors - Liu Yong Y

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

eman (Verified Customer) (11-26-2020)

this antibody works very well incubating either at room temperature or overnight2 dilutions are tested 1:1000 and 1:2000IF not tested yet

• Applications: Western Blot, Immunoprecipitation
• Primary Antibody Dilution: 1:1000 and 1:2000
• Cell Tissue Type: Hela cells

AEG-1/MTDH-Specific Antibody Western Blot,Immunoprecipitation validation (1:1000 and 1:2000 dilution) in Hela cells (Cat no:13860-1-AP)

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