Anticorps Polyclonal de lapin anti-MTF1

• Phare
• Validé par KD/KO

MTF1 Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (2)

Applications

WB, IHC, IF, IP, chIP, ELISA

Conjugaison

Non conjugué

Publications(36)

Avis (1)

N° de cat : 25383-1-AP

Synonymes

MTF 1, Transcription factor MTF 1, Transcription factor MTF-1, ZRF

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Galerie de données de validation

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  WB analysis of mouse liver using 25383-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 25383-1-AP (MTF1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse skeletal muscle using 25383-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 25383-1-AP (MTF1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 25383-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 25383-1-AP (MTF1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 25383-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 25383-1-AP (MTF1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 25383-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 25383-1-AP (MTF1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver using 25383-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 25383-1-AP (MTF1 Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human liver using 25383-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 25383-1-AP (MTF1 Antibody) at dilution of 1:200 (under 10x lens).

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Applications testées

• Résultats positifs en WB: tissu hépatique de souris, cellules Jurkat, tissu cardiaque de souris, tissu cérébral de souris, tissu de muscle squelettique de souris
• Résultats positifs en IHC: tissu hépatique humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 5 publications below
• WB: See 27 publications below
• IHC: See 5 publications below
• IF: See 3 publications below
• IP: See 1 publications below
• ChIP: See 5 publications below

Informations sur le produit

25383-1-AP cible MTF1 dans les applications de WB, IHC, IF, IP, chIP, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: rat, Humain, souris, fish
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: MTF1 Protéine recombinante Ag21848
• Nom complet: metal-regulatory transcription factor 1
• Masse moléculaire calculée: 753 aa, 81 kDa
• Poids moléculaire observé: 65-70 kDa, 100 kDa
• Numéro d’acquisition GenBank: BC014454
• Symbole du gène: MTF1
• Identification du gène (NCBI): 4520
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Metal regulatory transcription factor 1 (MTF1), contains six C2H2-type zinc finger and localizes in the nucleus. MTF1 is a 753 amino acid protein and calculated molecular weight is 81 kDa. We always dectected a 65-70 protein by western blot. MTF1 binds to the metal responsive element and activates the metallothionein I promoter.

Publications

Species	Application	Title
mouse,human	WB	Cancer Cell; Hypoxia inducible factor-1α drives cancer resistance to cuproptosis; Authors - Zhou Yang
human	WB	Adv Sci (Weinh); LncRNA MT1DP Aggravates Cadmium-Induced Oxidative Stress by Repressing the Function of Nrf2 and is Dependent on Interaction with miR-365.; Authors - Ming Gao ; KD Validated
human	WB	Nat Commun; Autophagy of the m6A mRNA demethylase FTO is impaired by low-level arsenic exposure to promote tumorigenesis.; Authors - Yan-Hong Cui ; KD Validated
fish	WB,IP	Environ Sci Technol; Zn Induces Lipophagy via the Deacetylation of Beclin1 and Alleviates Cu-Induced Lipotoxicity at Their Environmentally Relevant Concentrations.; Authors - Xiaolei Wei
	WB	Environ Sci Technol; Mitochondria-Dependent Oxidative Stress Mediates ZnO Nanoparticle (ZnO NP)-Induced Mitophagy and Lipotoxicity in Freshwater Teleost Fish.; Authors - Guang-Hui Chen
human	WB,IHC	Oxid Med Cell Longev; Ferroportin-Dependent Iron Homeostasis Protects against Oxidative Stress-Induced Nucleus Pulposus Cell Ferroptosis and Ameliorates Intervertebral Disc Degeneration In Vivo.; Authors - Saideng Lu

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

David (Verified Customer) (09-15-2025)

Concentration used 1:1000. Works pretty good. Strong clean signal

• Applications: Western Blot

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