Anticorps Polyclonal de lapin anti-MTF2

• Phare
• Validé par KD/KO

MTF2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, chIP, ELISA

Conjugaison

Non conjugué

Publications(28)

N° de cat : 16208-1-AP

Synonymes

PCL2, MTF 2, Metal-response element-binding transcription factor 2, Metal-response element DNA-binding protein M96, M96

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Galerie de données de validation

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  WB analysis of HepG2 using 16208-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 16208-1-AP (MTF2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis using 16208-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 16208-1-AP (MTF2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse ES cells and 293 cells using 16208-1-AP

  WB result of anti-MTF2 (16208-1-AP) from Kevin Corbit.

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  IP experiment of mouse skeletal muscle using 16208-1-AP

  IP result of anti-MTF2 (IP:16208-1-AP, 4ug; Detection:16208-1-AP 1:300) with mouse skeletal muscle tissue lysate 4000ug.

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  IHC staining of human brain using 16208-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 16208-1-AP (MTF2 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human brain using 16208-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 16208-1-AP (MTF2 antibody) at dilution of 1:50 (under 10x lens).

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  IF Staining of mouse ES cells using 16208-1-AP

  IF result of mouse ES cell From Kevin Corbit.

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Applications testées

• Résultats positifs en WB: cellules HepG2, cellules Jurkat, cellules SE de souris et cellules 293
• Résultats positifs en IP: tissu de muscle squelettique de souris
• Résultats positifs en IHC: tissu cérébral humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules SE de souris

Dilution recommandée

• Western Blot (WB): WB : 1:200-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 23 publications below
• IHC: See 3 publications below
• IF: See 1 publications below
• IP: See 1 publications below
• ChIP: See 5 publications below

Informations sur le produit

16208-1-AP cible MTF2 dans les applications de WB, IHC, IF/ICC, IP, chIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: MTF2 Protéine recombinante Ag9182
• Nom complet: metal response element binding transcription factor 2
• Masse moléculaire calculée: 536 aa, 61 kDa
• Poids moléculaire observé: 67 kDa, 60 kDa, 60-61 kDa
• Numéro d’acquisition GenBank: BC010013
• Symbole du gène: MTF2
• Identification du gène (NCBI): 22823
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

MTF2, also named as Metal-response element-binding transcription factor 2, is a 593 amino acid protein. It exists several isoforms and the range of the molecular weight of isoforms are about 55-60 kDa and 29 kDa. MTF2 contains 2 PHD-type zinc fingers and belongs to the Polycomblike family. MTF2 acts by binding to H3K36me3, a mark for transcriptional activation, and recruiting the PRC2 complex, leading to enhance PRC2 H3K27me3 methylation activity and regulates the transcriptional networks during embryonic stem cell self-renewal and differentiation.

Publications

Species	Application	Title
mouse	WB	Nature; Polycomb-like proteins link the PRC2 complex to CpG islands.; Authors - Haojie Li ; KO Validated
mouse	WB	Nat Genet; MTF2 recruits Polycomb Repressive Complex 2 by helical-shape-selective DNA binding.; Authors - Matteo Perino
human	WB	Nat Genet; RNA is essential for PRC2 chromatin occupancy and function in human pluripotent stem cells.; Authors - Yicheng Long
human	WB	Nat Cell Biol; Targeting Menin disrupts the KMT2A/B and polycomb balance to paradoxically activate bivalent genes; Authors - Christina E Sparbier
mouse	WB	Nat Struct Mol Biol; G-tract RNA removes Polycomb repressive complex 2 from genes.; Authors - Manuel Beltran
human	WB,ChIP	Mol Cell; Competition between PRC2.1 and 2.2 subcomplexes regulates PRC2 chromatin occupancy in human stem cells.; Authors - Daniel T Youmans

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