Anticorps Polyclonal de lapin anti-MTIF3

• Phare
• Validé par KD/KO

MTIF3 Polyclonal Antibody for IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (1)

Applications

WB, IP, IHC, ELISA

Conjugaison

Non conjugué

Publications

7

Avis (1)

N° de cat : 14219-1-AP

Synonymes

IF 3(Mt), IF 3mt, IF3(mt), IF3mt, MTIF3

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Galerie de données de validation

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  WB analysis using 14219-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14219-1-AP (MTIF3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human preadipocyte cells using 14219-1-AP

  The MTIF3 knockout (>80% KO efficiency as evaluated by Sanger sequencing) and wildtype (WT) human preadipocyte cells were lysed in 1% SDS. 10 μg total protein were subjected to SDS PAGE followed by western blot with 14219-1-AP (MTIF3 antibody) at dilution of 1:2000 incubated overnight at 4 oC. Date from Dr. Mi Huang.

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  IP experiment of HeLa using 14219-1-AP

  IP Result of anti-MTIF3 (IP:14219-1-AP, 4ug; Detection:14219-1-AP 1:500) with HeLa cells lysate 3200ug.

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  IHC staining of human liver using 14219-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 14219-1-AP (MTIF3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 14219-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 14219-1-AP (MTIF3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 14219-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 14219-1-AP (MTIF3 antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 14219-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 14219-1-AP (MTIF3 antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human testis using 14219-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 14219-1-AP (MTIF3 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human testis using 14219-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 14219-1-AP (MTIF3 Antibody) at dilution of 1:50 (under 40x lens).

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Applications testées

• Résultats positifs en WB: cellules A431, cellules HeLa, cellules HepG2, cellules HT-29
• Résultats positifs en IP: cellules HeLa
• Résultats positifs en IHC: tissu hépatique humain, tissu testiculaire de souris, tissu testiculaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 6 publications below

Informations sur le produit

14219-1-AP cible MTIF3 dans les applications de WB, IP, IHC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: MTIF3 Protéine recombinante Ag5457
• Nom complet: mitochondrial translational initiation factor 3
• Masse moléculaire calculée: 32 kDa
• Poids moléculaire observé: 29 kDa
• Numéro d’acquisition GenBank: BC046166
• Symbole du gène: MTIF3
• Identification du gène (NCBI): 219402
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

MTIF3, also named as DC38, belongs to the IF-3 family. MTIF3 encodes a 29 kDa protein that promotes formation of the initiation complex on the mitochondrial 55S ribosome, thereby playing an active role in initiation of translation. Like bacterial IF3, MTIF3 is believed to bind first to the small mitoribosomal subunit to keep it dissociated from the large subunit during initiation. After binding of MTIF3, mRNA and formylated initiator methionyl-tRNA (fMet-tRNAifMet) bind to the small mitoribosomal subunit. The large subunit then joins the small subunit to form an elongation-competent ribosome (PMID:20887776, 31350787).

Publications

Species	Application	Title
mouse	WB	Sci Adv; Fidelity of translation initiation is required for coordinated respiratory complex assembly.; Authors - Danielle L Rudler
mouse	WB	EMBO J; Stress signaling and cellular proliferation reverse the effects of mitochondrial mistranslation.; Authors - Nicola Ferreira
human	WB	Sci Rep; Initiation Factor 3 is Dispensable For Mitochondrial Translation in Cultured Human Cells.; Authors - Ivan V Chicherin
		Clin Immunol; Novel minor HLA DR associated antigens in type 1 diabetes.; Authors - Denise Müller
rat	WB	Sci Total Environ; Fluoride impairs mitochondrial translation by targeting miR-221-3p/c-Fos/RMND1 axis contributing to neurodevelopment defects; Authors - Dongjie Li
human	WB	Elife; Identification of a weight loss-associated causal eQTL in MTIF3 and the effects of MTIF3 deficiency on human adipocyte function; Authors - Mi Huang

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Mi (Verified Customer) (02-10-2023)

It works pretty well! We tried different antibodies from other suppliers, and this one gives a specific band, as we expected from the knockout samples. Although, we did observe some non-specific bands, having a knockout control would validate the target band.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: adipocytes