Anticorps Polyclonal de lapin anti-MTM1

MTM1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications(1)

Avis (1)

N° de cat : 13924-1-AP

Synonymes

Myotubularin, MTMX, EC:3.1.3.95, EC:3.1.3.64, CNM

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Galerie de données de validation

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  WB analysis of L02 using 13924-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 13924-1-AP (MTM1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 13924-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 13924-1-AP (MTM1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse skeletal muscle using 13924-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 13924-1-AP (MTM1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat skeletal muscle using 13924-1-AP

  Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue slide using 13924-1-AP (MTM1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat skeletal muscle using 13924-1-AP

  Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue slide using 13924-1-AP (MTM1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse skeletal muscle using 13924-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 13924-1-AP (MTM1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of L02 using 13924-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed L02 cells using MTM1 antibody (13924-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules L02, cellules Jurkat
• Résultats positifs en IHC: tissu de muscle squelettique de rat, tissu de muscle squelettique de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules L02,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below

Informations sur le produit

13924-1-AP cible MTM1 dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: porc
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: MTM1 Protéine recombinante Ag4940
• Nom complet: myotubularin 1
• Masse moléculaire calculée: 70 kDa
• Poids moléculaire observé: 65-70 kDa
• Numéro d’acquisition GenBank: BC030779
• Symbole du gène: MTM1
• Identification du gène (NCBI): 4534
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The MTM1(Myotubularin) gene encodes a protein that belongs to a family of putative tyrosine phosphatases. It is required for muscle cell differentiation. The protein is also a potent phosphatidylinositol 3-phosphate (PI3P) phosphatase, which is a 66 kDa ubiquitous protein that is found mutated in the X-linked genetic disorder myotubular myopathy(XLMTM).MTM1 could therefore act as a regulator of the highly active phosphoinositide (PI) metabolism in cells, where interconversions of the eight PIs described so far is controlled by a set of specific kinases and phosphatases(PMID:14660569). MTM1 has two isoforms with the molecular mass of 66kDa and 70 kDa.

Publications

Species	Application	Title
pig	WB	PLoS Pathog; Pseudorabies virus inhibits progesterone-induced inactivation of TRPML1 to facilitate viral entry; Authors - Bing-Qian Su

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Siting (Verified Customer) (01-28-2020)

This antibody worked for western blot.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Mouse heart tissue

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