Anticorps Polyclonal de lapin anti-MTM1

MTM1 Polyclonal Antibody for IF, IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Avis (1)

N° de cat : 13924-1-AP

Synonymes

CG2, CNM, MTM1, MTMX, Myotubularin, myotubularin 1, XLMTM

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Galerie de données de validation

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  WB analysis of L02 using 13924-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 13924-1-AP (MTM1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 13924-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 13924-1-AP (MTM1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of rat skeletal muscle using 13924-1-AP

  Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue slide using 13924-1-AP (MTM1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat skeletal muscle using 13924-1-AP

  Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue slide using 13924-1-AP (MTM1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse skeletal muscle using 13924-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 13924-1-AP (MTM1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse skeletal muscle using 13924-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 13924-1-AP (MTM1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of L02 using 13924-1-AP

  Immunofluorescent analysis of L02 cells using 13924-1-AP (MTM1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules L02, cellules Jurkat
• Résultats positifs en IHC: tissu de muscle squelettique de rat, tissu de muscle squelettique de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules L02

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

13924-1-AP cible MTM1 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: MTM1 Protéine recombinante Ag4940
• Nom complet: myotubularin 1
• Masse moléculaire calculée: 70 kDa
• Poids moléculaire observé: 65-70 kDa
• Numéro d’acquisition GenBank: BC030779
• Symbole du gène: MTM1
• Identification du gène (NCBI): 4534
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The MTM1(Myotubularin) gene encodes a protein that belongs to a family of putative tyrosine phosphatases. It is required for muscle cell differentiation. The protein is also a potent phosphatidylinositol 3-phosphate (PI3P) phosphatase, which is a 66 kDa ubiquitous protein that is found mutated in the X-linked genetic disorder myotubular myopathy(XLMTM).MTM1 could therefore act as a regulator of the highly active phosphoinositide (PI) metabolism in cells, where interconversions of the eight PIs described so far is controlled by a set of specific kinases and phosphatases(PMID:14660569). MTM1 has two isoforms with the molecular mass of 66kDa and 70 kDa.

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Siting (Verified Customer) (01-28-2020)

This antibody worked for western blot.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Mouse heart tissue