Anticorps Polyclonal de lapin anti-MTR

MTR Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications(15)

N° de cat : 25896-1-AP

Synonymes

Methionine synthase, 5-methyltetrahydrofolate--homocysteine methyltransferase, cblG, Cobalamin-dependent methionine synthase, EC:2.1.1.13

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Galerie de données de validation

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  WB analysis using 25896-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 25896-1-AP (MTR antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of A375 using 25896-1-AP

  A375 cells were subjected to SDS PAGE followed by western blot with 25896-1-AP (MTR Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse pancreas using 25896-1-AP

  mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 25896-1-AP (MTR Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human cervical cancer using 25896-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 25896-1-AP (MTR antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human cervical cancer using 25896-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 25896-1-AP (MTR antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human heart using 25896-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 25896-1-AP (MTR Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human heart using 25896-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 25896-1-AP (MTR Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human pancreas using 25896-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 25896-1-AP (MTR Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human pancreas using 25896-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 25896-1-AP (MTR Antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of A375 using 25896-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed A375 cells using 25896-1-AP (MTR antibody), at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules A375, cellules HEK-293, cellules HepG2, cellules L02, tissu pancréatique de souris
• Résultats positifs en IHC: tissu de cancer du col de l'utérus humain, tissu cardiaque humain, tissu pancréatique humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules A375,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:8000
• Immunohistochimie (IHC): IHC : 1:250-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 13 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Informations sur le produit

25896-1-AP cible MTR dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: MTR Protéine recombinante Ag23156
• Nom complet: 5-methyltetrahydrofolate-homocysteine methyltransferase
• Poids moléculaire observé: 140 kDa
• Numéro d’acquisition GenBank: BC130616
• Symbole du gène: MTR
• Identification du gène (NCBI): 4548
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

MTR (Methionine synthase), one of two B12-dependent mammalian enzymes, catalyzes a methyl transfer from methyltetrahydrofolate to homocysteine, generating methionine and tetrahydrofolate (PMID: 9587027, 9013615). Methionine synthase levels in mammalian cells are influenced by folate status, and the ability of cells to grow in the absence of methionine is partly rate-limited by synthase activity levels. MTR loss depletes cells and tumors of reduced folate species (PMID: 34799699)

Publications

Species	Application	Title
human		Cell Metab; Transsulfuration Activity Can Support Cell Growth upon Extracellular Cysteine Limitation.; Authors - Jiajun Zhu
human	WB	Mol Cell; mTORC1 stimulates cell growth through SAM synthesis and m6A mRNA-dependent control of protein synthesis.; Authors - Elodie Villa
human	WB	J Pineal Res; Melatonin modulates metabolic remodeling in HNSCC by suppressing MTHFD1L-formate axis.; Authors - Li Cui
mouse	WB	Clin Epigenetics; A transgenic mice model of retinopathy of cblG-type inherited disorder of one-carbon metabolism highlights epigenome-wide alterations related to cone photoreceptor cells development and retinal metabolism; Authors - Karim Matmat
mouse	WB	Nutrients; Mild Choline Deficiency and MTHFD1 Synthetase Deficiency Interact to Increase Incidence of Developmental Delays and Defects in Mice.; Authors - Karen E Christensen
human	WB	iScience; Linkage of methionine addiction, histone lysine hypermethylation, and malignancy.; Authors - Jun Yamamoto

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