Anticorps Polyclonal de lapin anti-MTRF1L

MTRF1L Polyclonal Antibody for WB,ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, ELISA

Conjugaison

Non conjugué

Publications

3

N° de cat : 16694-1-AP

Synonymes

MRF1L, mtRF1a, MTRF1L

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Galerie de données de validation

• 

  WB analysis of HEK-293 using 16694-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 16694-1-AP (MTRF1L Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 16694-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 16694-1-AP (MTRF1L Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 16694-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 16694-1-AP (MTRF1L Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 16694-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 16694-1-AP (MTRF1L Antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 16694-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 16694-1-AP (MTRF1L Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 16694-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 16694-1-AP (MTRF1L Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 16694-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 16694-1-AP (MTRF1L Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules HeLa, cellules HepG2

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 3 publications below

Informations sur le produit

16694-1-AP cible MTRF1L dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: MTRF1L Protéine recombinante Ag10081
• Nom complet: mitochondrial translational release factor 1-like
• Masse moléculaire calculée: 380 aa, 44 kDa
• Poids moléculaire observé: 40-46 kDa
• Numéro d’acquisition GenBank: BC011873
• Symbole du gène: MTRF1L
• Identification du gène (NCBI): 54516
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

MTRF1L, also named as Peptide chain release factor 1-like, mitochondrial, is a 380 amino acid protein, which belongs to the prokaryotic/mitochondrial release factor family and locates in the mitochondrial. MTRF1L directs the termination of translation in response to the peptide chain termination codons UAA and UAG. MTRF1L is expressed in skeletal muscle.

Publications

Species	Application	Title
mouse	WB	Biochem Biophys Res Commun; Muscle denervation reduces mitochondrial biogenesis and mitochondrial translation factor expression in mice.; Authors - Takumi Yokokawa
mouse	WB	Physiol Rep; Exercise-induced mitochondrial biogenesis coincides with the expression of mitochondrial translation factors in murine skeletal muscle.; Authors - Takumi Yokokawa
rat	WB	Sci Total Environ; Fluoride impairs mitochondrial translation by targeting miR-221-3p/c-Fos/RMND1 axis contributing to neurodevelopment defects; Authors - Dongjie Li