Anticorps Polyclonal de lapin anti-MTX1

• Phare
• Validé par KD/KO

MTX1 Polyclonal Antibody for WB, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (2)

Applications

WB, IP, ELISA

Conjugaison

Non conjugué

Publications(4)

N° de cat : 15529-1-AP

Synonymes

metaxin 1, MTX, MTX1, MTXN

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Galerie de données de validation

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  WB analysis using 15529-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15529-1-AP (MTX1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis using 15529-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15529-1-AP (MTX1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IP experiment of MCF-7 using 15529-1-AP

  IP result of anti-MTX1 (IP:15529-1-AP, 4ug; Detection:15529-1-AP 1:700) with MCF-7 cells lysate 1280ug.

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Applications testées

• Résultats positifs en WB: cellules HepG2, cellules A549, cellules HeLa, cellules Jurkat
• Résultats positifs en IP: cellules MCF-7

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 4 publications below
• IP: See 1 publications below

Informations sur le produit

15529-1-AP cible MTX1 dans les applications de WB, IP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: MTX1 Protéine recombinante Ag7685
• Nom complet: metaxin 1
• Masse moléculaire calculée: 51 kDa
• Poids moléculaire observé: 35 kDa
• Numéro d’acquisition GenBank: BC001906
• Symbole du gène: MTX1
• Identification du gène (NCBI): 4580
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

METAXINs are proteins located in the outer membrane of the mammalian mitochondrion. METAXIN 1 was characterized firstly and was found to play a role in the import of mitochondrial precursor proteins. It may also play a role in the assembly of b-barrel proteins into the outer membrane METAXIN1 was reported to be essential for survival of the postimplantation mouse embryo. METAXIN 1 has multiple transcripts. Several isoforms of METAXIN 1 exist due to the alternative splicing, with the predicted MW of 51, 48 and 36 kDa, respectively. Metaxin 1 interacts with metaxin 2, a novel related protein associated with the mammalian mitochondrial outer membrane and form complex in the mitochondrion outer membrane.

Publications

Species	Application	Title
human	WB,IP	Int J Biol Sci; Genome-scale CRISPRa screening identifies MTX1 as a contributor for sorafenib resistance in hepatocellular carcinoma by augmenting autophagy.; Authors - Li Li ; KD Validated
mouse	WB	Hepatology; Loss of Sam50 in hepatocytes induces cardiolipin-dependent mitochondrial membrane remodeling to trigger mtDNA release and liver injury; Authors - Li Chen
human	WB	J Cell Sci; Myo19 tethers mitochondria to endoplasmic reticulum-associated actin to promote mitochondrial fission; Authors - Stephen M Coscia ; KD Validated
rat	WB	NPJ Parkinsons Dis; Disruption of axonal transport in Parkinson's disease: the role of pathological α-Syn and AMPK/p38 MAPK signaling; Authors - Xiaoman Yang

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