Anticorps Polyclonal de lapin anti-MUC16,CA125

• Phare
• Validé par KD/KO

MUC16,CA125 Polyclonal Antibody for IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IP, IHC, ELISA

Conjugaison

Non conjugué

Publications

6

N° de cat : 20077-1-AP

Synonymes

CA 125, CA125, MUC 16, MUC16, MUC16,CA125, Mucin 16

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Galerie de données de validation

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  WB analysis of HeLa using 20077-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 20077-1-AP (MUC16,CA125 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 20077-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 20077-1-AP (MUC16,CA125 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 20077-1-AP

  IP Result of anti-MUC16,CA125 (IP:20077-1-AP, 4ug; Detection:20077-1-AP 1:600) with HeLa cells lysate 1200ug.

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  IHC staining of human ovary tumor using 20077-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 20077-1-AP (MUC16,CA125 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human ovary tumor using 20077-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 20077-1-AP (MUC16,CA125 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules HeLa
• Résultats positifs en IP: cellules HeLa
• Résultats positifs en IHC: tissu de tumeur ovarienne humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 5 publications below
• IHC: See 1 publications below

Informations sur le produit

20077-1-AP cible MUC16,CA125 dans les applications de WB, IP, IHC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: mucin 16, cell surface associated
• Masse moléculaire calculée: 1519 kDa
• Poids moléculaire observé: 90-100 kDa
• Numéro d’acquisition GenBank: NM_024690
• Symbole du gène: MUC16
• Identification du gène (NCBI): 94025
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

MUC16, also named as CA125, is thought to provide a protective, lubricating barrier against particles and infectious agents at mucosal surfaces. It is a serum marker that is used routinely in gynecologic practice to monitor patients with ovarian cancer. Most biochemical studies have concluded that MUC16 is a high molecular mass glycoprotein, although estimates of its size range from 200 to 2000 kDa with smaller "subunits" being described by some investigators. The antibody recognizes the C-term of MUC16.

Publications

Species	Application	Title
human	IHC	Cancer Sci; Carcinoembryonic antigen as a specific glycoprotein ligand of rBC2LCN lectin on pancreatic ductal adenocarcinoma cells.; Authors - Tomoaki Furuta
human	WB	Toxicology; Necroptosis contributes to airborne particulate matter-induced ocular surface injury.; Authors - Kexin Shi
human	WB	Int J Oncol; Lewis antigen‑negative pancreatic cancer: An aggressive subgroup.; Authors - Chen Liu
human	WB	Biochem Biophys Res Commun; MUC16 mutation predicts a favorable clinical outcome and correlates decreased Warburg effect in gastric cancer.; Authors - Huijin Zhao
human	WB	J Exp Clin Cancer Res; Dysregulation and prometastatic function of glycosyltransferase C1GALT1 modulated by cHP1BP3/ miR-1-3p axis in bladder cancer.; Authors - Zengqi Tan ; KD Validated
human	WB	Cancers (Basel); Protein Panel of Serum-Derived Small Extracellular Vesicles for the Screening and Diagnosis of Epithelial Ovarian Cancer; Authors - Huiling Lai

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