Anticorps Polyclonal de lapin anti-MUC20

MUC20 Polyclonal Antibody for WB, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IP, ELISA

Conjugaison

Non conjugué

Publications(1)

N° de cat : 13380-1-AP

Synonymes

KIAA1359, MUC 20, MUC20, Mucin 20

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Galerie de données de validation

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  WB analysis of human liver using 13380-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 13380-1-AP (MUC20 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of human kidney using 13380-1-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 13380-1-AP (MUC20 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse liver using 13380-1-AP

  IP result of anti-MUC20 (IP:13380-1-AP, 4ug; Detection:13380-1-AP 1:300) with mouse liver tissue lysate 6000 ug.

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Applications testées

• Résultats positifs en WB: tissu hépatique humain, tissu rénal humain
• Résultats positifs en IP: tissu hépatique de souris,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below

Informations sur le produit

13380-1-AP cible MUC20 dans les applications de WB, IP, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: MUC20 Protéine recombinante Ag4205
• Nom complet: mucin 20, cell surface associated
• Masse moléculaire calculée: 55 kDa, 71 kDa
• Poids moléculaire observé: 70-75 kDa
• Numéro d’acquisition GenBank: BC029267
• Symbole du gène: MUC20
• Identification du gène (NCBI): 200958
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

MUC20 contains serine, threonine, and proline-rich repeats in its extracellular domain and is likely to be extensively O-glycosylated. MUC20 blocks GRB2 recruitment to MET thus suppressing the GRB2-RAS pathway and inhibiting HGF-induced proliferation of MMP1 and MMP9 expression (PMID: 15314156). MUC20 is highly expressed in the kidney and localized in the proximal tubules but not in the glomerulus or distal tubules(PMID: 14565953). MUC20 was detected in most of the male urogenital tract epithelia, except for epididymis(PMID: 16997931). The expression of MUC20 is up-regulated in the kidneys of patients with immunoglobulin A nephropathy, in an animal model of lupus nephritis, and in mice with acute renal injury caused by cisplatin administration or unilateral ureteral obstruction(PMID: 14565953).

Publications

Species	Application	Title
human	WB	J Exp Clin Cancer Res; MUC20 regulated by extrachromosomal circular DNA attenuates proteasome inhibitor resistance of multiple myeloma by modulating cuproptosis; Authors - Xiaobin Wang

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