Anticorps Polyclonal de lapin anti-MUM1

MUM1 Polyclonal Antibody for IP, WB,ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (1)

Applications

WB, IP, ELISA

Conjugaison

Non conjugué

Publications

1

Avis (1)

N° de cat : 12682-1-AP

Synonymes

EXPAND1, HSPC211, MUM 1, MUM1, Protein expandere, PWWP3A

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Galerie de données de validation

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  WB analysis using 12682-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12682-1-AP (MUM1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HT-1080 using 12682-1-AP

  HT-1080 cells were subjected to SDS PAGE followed by western blot with 12682-1-AP (MUM1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HT-1080 using 12682-1-AP

  HT-1080 cells were subjected to SDS PAGE followed by western blot with 12682-1-AP (MUM1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 12682-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 12682-1-AP (MUM1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of Jurkat using 12682-1-AP

  IP Result of anti-MUM1 (IP:12682-1-AP, 4ug; Detection:12682-1-AP 1:300) with Jurkat cells lysate 4000ug.

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules HT-1080, cellules Jurkat
• Résultats positifs en IP: cellules Jurkat

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below

Informations sur le produit

12682-1-AP cible MUM1 dans les applications de WB, IP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: MUM1 Protéine recombinante Ag3371
• Nom complet: melanoma associated antigen (mutated) 1
• Masse moléculaire calculée: 18 kDa, 79 kDa
• Poids moléculaire observé: 70-80 kDa
• Numéro d’acquisition GenBank: BC008098
• Symbole du gène: MUM1
• Identification du gène (NCBI): 84939
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

MUM1, also named as EXPAND1, is an architectural component of the chromatin, which in response to DNA damage serves as an accessory factor to promote cell survival. It is a 53BP1-associated DNA damage-responsive factor. MUM1/EXPAND1 is different to another protein IRF4/MUM1.

Publications

Species	Application	Title
mouse	WB	Nature; Post-translational control of beige fat biogenesis by PRDM16 stabilization; Authors - Qiang Wang

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Aurelie (Verified Customer) (09-01-2019)

Lysats (20µg) from Mouse cortex were loaded in a 8% SDS-PAGE and immunoblotted using anti-MUM1 antibody ( 1/1000) over-night at 4°C.

• Applications: Western Blot,
• Primary Antibody Dilution: 1/1000
• Cell Tissue Type: Mouse Cortex

MUM1 Antibody Western Blot, validation (1/1000 dilution) in Mouse Cortex (Cat no:12682-1-AP)