Anticorps Recombinant de lapin anti-Methylmalonyl Coenzyme A mutase/MUT

Methylmalonyl Coenzyme A mutase/MUT Recombinant Antibody for IHC, IF/ICC, FC (Intra), ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

IHC, IF/ICC, FC (Intra), ELISA

Conjugaison

Non conjugué

CloneNo.

242198A3

N° de cat : 84879-6-RR

Synonymes

MUT, EC:5.4.99.2, MCM, Methylmalonyl CoA isomerase, Methylmalonyl Coenzyme A mutase

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Galerie de données de validation

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  IHC staining of Human Hepatocellular cancer using 84879-6-RR

  Immunohistochemical analysis of paraffin-embedded Human Hepatocellular cancer slide using 84879-6-RR (Methylmalonyl Coenzyme A mutase/MUT antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of Human Hepatocellular cancer using 84879-6-RR

  Immunohistochemical analysis of paraffin-embedded Human Hepatocellular cancer slide using 84879-6-RR (Methylmalonyl Coenzyme A mutase/MUT antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 84879-6-RR

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using Methylmalonyl Coenzyme A mutase/MUTantibody (84879-6-RR, Clone: 242198A3 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  FC experiment of HepG2 using 84879-6-RR

  1x10^6 HepG2 cells were intracellularly stained with 0.25 ug Methylmalonyl Coenzyme A mutase/MUT Recombinant Antibody (84879-6-RR, Clone:242198A3) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  FC experiment of A431 using 84879-6-RR

  1x10^6 A431 cells were intracellularly stained with 0.25 ug Methylmalonyl Coenzyme A mutase/MUT Recombinant Antibody (84879-6-RR, Clone:242198A3) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en IHC: Human Hepatocellular cancer, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HepG2,
• Résultats positifs en FC (Intra): cellules A431, cellules HepG2

Dilution recommandée

• Immunohistochimie (IHC): IHC : 1:250-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:250-1:1000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

84879-6-RR cible Methylmalonyl Coenzyme A mutase/MUT dans les applications de IHC, IF/ICC, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Methylmalonyl Coenzyme A mutase/MUT Protéine recombinante Ag10523
• Nom complet: methylmalonyl Coenzyme A mutase
• Masse moléculaire calculée: 750 aa, 83 kDa
• Poids moléculaire observé: 78 kDa
• Numéro d’acquisition GenBank: BC016282
• Symbole du gène: MUT
• Identification du gène (NCBI): 4594
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Methylmalonyl Coenzyme A mutase (MUT) is an enzyme that plays a crucial role in the metabolism of certain amino acids and fatty acids. Mutations in the MUT gene can lead to methylmalonic acidemia, a metabolic disorder characterized by the accumulation of toxic compounds such as methylmalonyl-CoA and propionyl-CoA. This condition can cause severe health issues including developmental delays, metabolic acidosis, and neurological problems. MUT is essential for maintaining normal metabolic processes and its dysfunction can have significant health implications, highlighting its importance in both basic metabolism and clinical medicine.

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