Anticorps Polyclonal de lapin anti-MYBPC1

MYBPC1 Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications(3)

Avis (1)

N° de cat : 22900-1-AP

Synonymes

C-protein, skeletal muscle slow isoform, MYBPCS, Myosin-binding protein C, slow-type, Slow MyBP C, Slow MyBP-C

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Galerie de données de validation

• 

  WB analysis of mouse skeletal muscle using 22900-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 22900-1-AP (MYBPC1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse skeletal muscle using 22900-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 22900-1-AP (MYBPC1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse pancreas using 22900-1-AP

  mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 22900-1-AP (MYBPC1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human skeletal muscle using 22900-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 22900-1-AP (MYBPC1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human skeletal muscle using 22900-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 22900-1-AP (MYBPC1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of mouse skeletal muscle using 22900-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 22900-1-AP (MYBPC1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse bladder using 22900-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse bladder tissue slide using 22900-1-AP (MYBPC1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse heart using 22900-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 22900-1-AP (MYBPC1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human heart using 22900-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 22900-1-AP (MYBPC1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human heart using 22900-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 22900-1-AP (MYBPC1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human bladder using 22900-1-AP

  Immunohistochemical analysis of paraffin-embedded human bladder using 22900-1-AP (MYBPC1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human bladder using 22900-1-AP

  Immunohistochemical analysis of paraffin-embedded human bladder using 22900-1-AP (MYBPC1 antibody) at dilution of 1:50 (under 40x lens).

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Applications testées

• Résultats positifs en WB: tissu de muscle squelettique de souris, tissu pancréatique de souris
• Résultats positifs en IHC: tissu de muscle squelettique de souris, tissu cardiaque de souris, tissu cardiaque humain, tissu de muscle squelettique humain, tissu vésical de souris, tissu vésical humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below
• IHC: See 2 publications below

Informations sur le produit

22900-1-AP cible MYBPC1 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: myosin binding protein C, slow type
• Masse moléculaire calculée: 128 kDa
• Poids moléculaire observé: 135-140 kDa
• Numéro d’acquisition GenBank: NM_002465
• Symbole du gène: MYBPC1
• Identification du gène (NCBI): 4604
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

MYBPC1, also named as MYBPCS, belongs to the immunoglobulin superfamily and MyBP family. It is a thick filament-associated protein located in the crossbridge region of vertebrate striated muscle a bands. In vitro it binds MHC, F-actin and native thin filaments, and modifies the activity of actin-activated myosin ATPase. It may modulate muscle contraction or may play a more structural role. This antibody is specific to MYBPC1.

Publications

Species	Application	Title
mouse,human	IHC	Mol Psychiatry; FXR1 regulation of parvalbumin interneurons in the prefrontal cortex is critical for schizophrenia-like behaviors.; Authors - Minjie Shen
rat	WB	Dokl Biochem Biophys; Early Deсline in Rat Soleus Passive Tension with Hindlimb Unloading: Inactivation of Cross-bridges or Activation of Calpains?; Authors - I O Petrova
mouse	IHC	Adv Biol (Weinh); Glucose-Responsive Fibrin Hydrogel-Based Multimodal Nucleic Acid Delivery System; Authors - Mangesh Morey

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Sirsendu (Verified Customer) (06-19-2020)

Used for immunohistochemistry of db/db mice skin tissue after wound healing.Please provide amazon UK gift (£20)

• Applications: Immunohistochemistry
• Primary Antibody Dilution: 1:200
• Cell Tissue Type: Mice skin tissue

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