Anticorps Monoclonal anti-MYBPC3

MYBPC3 Monoclonal Antibody for WB, IHC, IF-P, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, Lapin, porc, rat, souris

Applications

WB, IHC, IF-P, ELISA

Conjugaison

Non conjugué

CloneNo.

3C8F8

N° de cat : 67608-1-Ig

Synonymes

3C8F8, Cardiac MyBP C, Cardiac MyBP-C, C-protein, cardiac muscle isoform, MYBPC 3

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Galerie de données de validation

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  WB analysis of mouse heart using 67608-1-Ig

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 67608-1-Ig (MYBPC3 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  WB analysis of H9C2 using 67608-1-Ig

  H9C2 cells were subjected to SDS PAGE followed by western blot with 67608-1-Ig (MYBPC3 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rabbit heart using 67608-1-Ig

  rabbit heart tissue were subjected to SDS PAGE followed by western blot with 67608-1-Ig (MYBPC3 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of pig heart using 67608-1-Ig

  pig heart tissue were subjected to SDS PAGE followed by western blot with 67608-1-Ig (MYBPC3 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human heart using 67608-1-Ig

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 67608-1-Ig (MYBPC3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat heart using 67608-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat heart tissue slide using 67608-1-Ig (MYBPC3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of rat heart using 67608-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed rat heart tissue using MYBPC3 antibody (67608-1-Ig, Clone: 3C8F8 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Applications testées

• Résultats positifs en WB: tissu cardiaque de souris, cellules H9C2, tissu cardiaque de lapin, tissu cardiaque de porc
• Résultats positifs en IHC: tissu cardiaque humain, tissu cardiaque de rat; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF-P: tissu cardiaque de rat,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)-P: IF-P : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

67608-1-Ig cible MYBPC3 dans les applications de WB, IHC, IF-P, ELISA et montre une réactivité avec des échantillons Humain, Lapin, porc, rat, souris

• Réactivité: Humain, Lapin, porc, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: MYBPC3 Protéine recombinante Ag15848
• Nom complet: myosin binding protein C, cardiac
• Masse moléculaire calculée: 1274 aa, 141 kDa
• Poids moléculaire observé: 140-150 kDa
• Numéro d’acquisition GenBank: BC151211
• Symbole du gène: MYBPC3
• Identification du gène (NCBI): 4607
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

MYBPC3 belongs to the immunoglobulin superfamily and MyBP family. MYBPC3 located in the crossbridge region of vertebrate striated muscle a bands. In vitro it binds MHC, F-actin and native thin filaments, and modifies the activity of actin-activated myosin ATPase. It may modulate muscle contraction or may play a more structural role. Defects in MYBPC3 are the cause of cardiomyopathy familial hypertrophic type 4 (CMH4).

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