Anticorps Monoclonal anti-MYC tag

MYC tag Monoclonal Antibody for WB, IF/ICC, FC (Intra), IP, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Protéine recombinante et plus (2)

Applications

WB, IF/ICC, FC (Intra), IP, CoIP, ChIP, ELISA

Conjugaison

Non conjugué

Publications(328)

CloneNo.

1A5A2

Avis (8)

N° de cat : 60003-2-Ig

Synonymes

myc, EQKLISEEDL, MYC-tag

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• Galerie de données de validation
• Proteintech vs. Other Vendors

• 

  WB analysis using 60003-2-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 60003-2-Ig (MYC tag antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of recombinant proein using 60003-2-Ig

  Western blot of Myc-tagged fusion protein with anti-Myc-tag (60003-2-Ig) at various dilutions.

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  IP experiment of Transfected HEK-293 using 60003-2-Ig

  IP result of anti-MYC tag (IP:60003-2-Ig, 4ug; Detection:60003-2-Ig 1:10000) with Transfected HEK-293 cells lysate 300ug.

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  IF Staining of Transfected HEK-293 using 60003-2-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed Transfected HEK-293 cells using MYC tag antibody (60003-2-Ig, Clone: 1A5A2 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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  FC experiment of HEK-293 transfected with GFP-MYC using 60003-2-Ig

  1x10^6 HEK-293 transfected with GFP-MYC were intracellularly stained with 0.25 ug MYC tag Monoclonal antibody (60003-2-Ig, Clone:1A5A2) and Multi-rAb CoraLite® Plus 647-Goat Anti-Mouse Recombinant Secondary Antibody (H+L)(RGAM005). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: Transfected HEK-293T cells, protéine recombinante
• Résultats positifs en IP: cellules HEK-293 transfectées
• Résultats positifs en IF/ICC: cellules HEK-293 transfectées,
• Résultats positifs en FC (Intra): cellules HEK-293 transfectées,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)/ICC: IF/ICC : 1:500-1:2000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.50 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 227 publications below
• IF: See 24 publications below
• IP: See 76 publications below
• CoIP: See 50 publications below
• ChIP: See 1 publications below

Informations sur le produit

60003-2-Ig cible MYC tag dans les applications de WB, IF/ICC, FC (Intra), IP, CoIP, ChIP, ELISA et montre une réactivité avec des échantillons Protéine recombinante

• Réactivité: Protéine recombinante
• Réactivité citée: Humain, porc
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: Myc tag
• Symbole du gène: Myc tag
• Identification du gène (NCBI): 99
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. The c-Myc tag corresponds to amino acid residues(EQKLISEEDL) of the human c-Myc protein. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits. Myc-Tag mouse mAb detects recombinant proteins containing the Myc tag. The antibody recognizes the Myc-tag EQKLISEEDL fused to either the amino- or carboxy- terminus of targeted proteins.

Publications

Species	Application	Title
	WB	Nature; Promotion of DNA end resection by BRCA1-BARD1 in homologous recombination; Authors - Sameer Salunkhe
		Cell Res; Mannose antagonizes GSDME-mediated pyroptosis through AMPK activated by metabolite GlcNAc-6P; Authors - Yuan-Li Ai
	WB	Cell Discov; Glc7/PP1 dephosphorylates histone H3T11 to regulate autophagy and telomere silencing in response to nutrient availability; Authors - Xinyu Zhang
	WB	Nat Cancer; Lymphatic endothelial-like cells promote glioblastoma stem cell growth through cytokine-driven cholesterol metabolism; Authors - Linjie Zhao
	WB	Protein Cell; BiFC and FACS-based CRISPR screening revealed that QKI promotes PABPN1 LLPS in colorectal cancer cells; Authors - Mengxia Li
	CoIP	Nat Commun; Phosphoglycerate dehydrogenase activates PKM2 to phosphorylate histone H3T11 and attenuate cellular senescence; Authors - Yinsheng Wu

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

VASUDEVARAO (Verified Customer) (07-23-2025)

The antibody works very well at the recommended dilutions and performs excellently in both immunoprecipitation and immunoblotting applications.

• Applications: Western Blot, Immunohistochemistry, Immunofluorescence, Immunoprecipitation, Cell culture, Protein purification
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: Breast cancer cells

FH

RASHMI (Verified Customer) (07-26-2024)

Used this product, highly recommended

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: HEK-293

FH

Sarah (Verified Customer) (10-27-2023)

works well at low dilution for WB and worked for immunoprecipitation

• Applications: Western Blot, Immunoprecipitation
• Primary Antibody Dilution: 1:10,000
• Cell Tissue Type: 293T

FH

manohar (Verified Customer) (07-10-2023)

Used Nitrocellulose membrane with 5% milk as blocking, antibody diluted in 1% milk.

• Applications: Western Blot, Immunofluorescence
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: HEK293, Neuronal stem cells

FH

Amy (Verified Customer) (03-05-2023)

Clean bands for detecting over expressed Myc-tagged proteins in HEK293T cell lysates.

• Applications: Western Blot
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: HEK293T

MYC tag Antibody Western Blot validation (1:500 dilution) in HEK293T (Cat no:60003-2-Ig)

FH

Isabelle Cristine (Verified Customer) (01-14-2022)

The antibody works generally well for the detection of MYC-tagged proteins.

• Applications: Western Blot
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: HEK293 cells

MYC tag Antibody Western Blot validation (1:500 dilution) in HEK293 cells (Cat no:60003-2-Ig)

FH

Tom (Verified Customer) (02-10-2021)

HEK293T cells were transfected with a Myc-tagged construct. The 60003-2-Ig antibody (1:2000) resulted in a lot of background. An alternative antibody was used alongside this one, with a stronger band and less background being achieved.

• Applications: Western Blot
• Primary Antibody Dilution: 1 in 2000
• Cell Tissue Type: HEK293

MYC tag Antibody Western Blot validation (1 in 2000 dilution) in HEK293 (Cat no:60003-2-Ig)

FH

Alinda (Verified Customer) (10-09-2019)

This antibody was used at 1:1000 in HEK cells transfected with a myc-tagged insert cloned into an overxpression. Cells were blocked for 1 hr at room temp in 5%goat serum PBST.This antibody has a lot of background; another antibody from cell signalling was tested in parallel and performed much better

• Applications: Immunofluorescence,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HEK cells

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