Anticorps Monoclonal anti-MYC tag
MYC tag Monoclonal Antibody for IF, IP, WB, ELISA
Hôte / Isotype
Mouse / IgG1
Réactivité testée
Protéine recombinante et plus (5)
Applications
WB, IP, IF, CoIP, ChIP, ELISA
Conjugaison
Non conjugué
184
CloneNo.
1A5A2
N° de cat : 60003-2-Ig
Synonymes
"MYC tag Antibodies" Comparison
View side-by-side comparison of MYC tag antibodies from other vendors to find the one that best suits your research needs.
Applications testées
| Résultats positifs en WB | Protéine recombinante |
| Résultats positifs en IP | cellules HEK-293 transfectées |
| Résultats positifs en IF | cellules HEK-293 transfectées, |
Dilution recommandée
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunoprécipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunofluorescence (IF) | IF : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, check data in validation data gallery | |
Applications publiées
| WB | See 127 publications below |
| IF | See 18 publications below |
| IP | See 43 publications below |
| CoIP | See 28 publications below |
| ChIP | See 1 publications below |
Informations sur le produit
60003-2-Ig cible MYC tag dans les applications de WB, IP, IF, CoIP, ChIP, ELISA et montre une réactivité avec des échantillons Protéine recombinante
| Réactivité | Protéine recombinante |
| Réactivité citée | rat, Humain, levure, porc, souris |
| Hôte / Isotype | Mouse / IgG1 |
| Clonalité | Monoclonal |
| Type | Anticorps |
| Immunogène | Peptide |
| Nom complet | MYC tag |
| Symbole du gène | |
| Identification du gène (NCBI) | |
| Conjugaison | Non conjugué |
| Forme | Liquide |
| Méthode de purification | Purification par protéine G |
| Tampon de stockage | PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3 |
| Conditions de stockage | Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA. |
Informations générales
Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. The c-Myc tag corresponds to amino acid residues(EQKLISEEDL) of the human c-Myc protein. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits. Myc-Tag mouse mAb detects recombinant proteins containing the Myc tag. The antibody recognizes the Myc-tag EQKLISEEDL fused to either the amino- or carboxy- terminus of targeted proteins.
Protocole
| Product Specific Protocols | |
|---|---|
| IF protocol for MYC tag antibody 60003-2-Ig | Download protocol |
| IP protocol for MYC tag antibody 60003-2-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cell Discov Glc7/PP1 dephosphorylates histone H3T11 to regulate autophagy and telomere silencing in response to nutrient availability | ||
Nat Cancer Lymphatic endothelial-like cells promote glioblastoma stem cell growth through cytokine-driven cholesterol metabolism | ||
Nat Commun Phosphoglycerate dehydrogenase activates PKM2 to phosphorylate histone H3T11 and attenuate cellular senescence | ||
Nat Commun UV-B irradiation-activated E3 ligase GmILPA1 modulates gibberellin catabolism to increase plant height in soybean | ||
Am J Respir Crit Care Med Loss of DP1 Aggravates Vascular Remodeling in Pulmonary Arterial Hypertension via mTORC1 Signaling. | ||
Hepatology The novel tumor suppressor IRF2BP2 regulates Hippo pathway in liver cancer via a feedback loop. |
Avis
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Sarah (Verified Customer) (10-27-2023) | works well at low dilution for WB and worked for immunoprecipitation
|
FH manohar (Verified Customer) (07-10-2023) | Used Nitrocellulose membrane with 5% milk as blocking, antibody diluted in 1% milk.
|
FH Amy (Verified Customer) (03-05-2023) | Clean bands for detecting over expressed Myc-tagged proteins in HEK293T cell lysates.
 |
FH Isabelle Cristine (Verified Customer) (01-14-2022) | The antibody works generally well for the detection of MYC-tagged proteins.
 |
FH Tom (Verified Customer) (02-10-2021) | HEK293T cells were transfected with a Myc-tagged construct. The 60003-2-Ig antibody (1:2000) resulted in a lot of background. An alternative antibody was used alongside this one, with a stronger band and less background being achieved.
 |
FH Alinda (Verified Customer) (10-09-2019) | This antibody was used at 1:1000 in HEK cells transfected with a myc-tagged insert cloned into an overxpression. Cells were blocked for 1 hr at room temp in 5%goat serum PBST.This antibody has a lot of background; another antibody from cell signalling was tested in parallel and performed much better
|
