Anticorps Monoclonal anti-MYC tag

MYC tag Monoclonal Antibody for WB, IF/ICC

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Protéine recombinante

Applications

WB, IF/ICC

Conjugaison

CoraLite® Plus 750 Fluorescent Dye

CloneNo.

1A5A2

N° de cat : CL750-60003

Synonymes

myc, EQKLISEEDL

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Galerie de données de validation

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  WB analysis using CL750-60003

  HEK-293 cell lysates and Recombinant protein(Ag19172) were subjected to SDS PAGE followed by western blot with CL750-60003 (MYC tag antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IF Staining of Transfected HEK-293 using CL750-60003

  Immunofluorescent analysis of (-20°C Ethanol) fixed Transfected HEK-293 cells using CoraLite® Plus 750 MYC tag antibody (CL750-60003, Clone: 1A5A2 ) at dilution of 1:100.

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  FC experiment of HEK-293 transfected with GFP-MYC using CL750-60003

  1x10^6 HEK-293 transfected with GFP-MYC were intracellularly stained with 0.4 ug CoraLite® Plus 750-conjugated MYC tag Monoclonal antibody (CL750-60003, Clone:1A5A2). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: Protéine recombinante,
• Résultats positifs en IF/ICC: cellules HEK-293 transfectées,
• Résultats positifs en FC (Intra): cellules HEK-293 transfectées

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

CL750-60003 cible MYC tag dans les applications de WB, IF/ICC et montre une réactivité avec des échantillons Protéine recombinante

• Réactivité: Protéine recombinante
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: Myc tag
• Symbole du gène: Myc tag
• Identification du gène (NCBI): 99
• Conjugaison: CoraLite® Plus 750 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 755 nm / 780 nm
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
• Conditions de stockage: Stocker à -20 °C. Éviter toute exposition à la lumière. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. The c-Myc tag corresponds to amino acid residues(EQKLISEEDL) of the human c-Myc protein. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits. Myc-Tag mouse mAb detects recombinant proteins containing the Myc tag. The antibody recognizes the Myc-tag EQKLISEEDL fused to either the amino- or carboxy- terminus of targeted proteins.

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