Anticorps Monoclonal anti-SMMHC

SMMHC Monoclonal Antibody for IF, IHC, WB, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, Lapin, porc, rat, souris

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

5

CloneNo.

5C6D3

N° de cat : 60222-1-Ig

Synonymes

MYH11, Myosin 11, Myosin heavy chain 11, SMHC, SMMHC, SMM-HC, smooth muscle myosin heavy chain

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Galerie de données de validation

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  WB analysis using 60222-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 60222-1-Ig (SMMHC antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 60222-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 60222-1-Ig (SMMHC antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 60222-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 60222-1-Ig (SMMHC antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human ileum using 60222-1-Ig

  human ileum tissue were subjected to SDS PAGE followed by western blot with 60222-1-Ig (SMMHC antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast hyperplasia using 60222-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast hyperplasia tissue slide using 60222-1-Ig (SMMHC antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast hyperplasia using 60222-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast hyperplasia tissue slide using 60222-1-Ig (SMMHC antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast hyperplasia using 60222-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast hyperplasia tissue slide using 60222-1-Ig (SMMHC antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 60222-1-Ig

  Immunofluorescent analysis of HeLa cells using 60222-1-Ig (SMMHC antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG (H+L).

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Applications testées

• Résultats positifs en WB: tissu d'estomac de lapin, cellules HeLa, côlon de rat, côlon de souris, estomac de porc, gros intestin de lapin, rectum de porc, tissu d'iléon humain, tissu rectal de rat
• Résultats positifs en IHC: tissu d'hyperplasie mammaire humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:20000
• Immunohistochimie (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 4 publications below
• IF: See 4 publications below

Informations sur le produit

60222-1-Ig cible SMMHC dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, Lapin, porc, rat, souris

• Réactivité: Humain, Lapin, porc, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: SMMHC Protéine recombinante Ag13397
• Nom complet: myosin, heavy chain 11, smooth muscle
• Masse moléculaire calculée: 1938 aa, 224 kDa
• Poids moléculaire observé: 220 kDa
• Numéro d’acquisition GenBank: BC101677
• Symbole du gène: MYH11
• Identification du gène (NCBI): 4629
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

SMMHC (smooth muscle myosin heavy chain; MYH11) is a contractile protein of smooth muscle cells. It is specifically expressed in cells derived from smooth muscle lineages. SMMHC is used as vascular smooth muscle cell (vSMC) contractile marker. It is also an excellent marker for myoepithelial cells, with no or few cross-reaction with myofibroblasts, thus very useful in the evaluation of stromal invasion.

Publications

Species	Application	Title
rat	IF	Biochim Biophys Acta Mol Basis Dis; Methamphetamine induces thoracic aortic aneurysm/dissection through C/EBPβ.; Authors - Bao-Ying Luo
mouse	WB,IF	Atherosclerosis; Nrf2 deficiency attenuates atherosclerosis by reducing LOX-1-mediated proliferation and migration of vascular smooth muscle cells.; Authors - Hongliang Li
rat	WB	Nutr Metab Cardiovasc Dis; Effects of carotid baroreceptor stimulation on aortic remodeling in obese rats.; Authors - Qiao Yu
mouse	IF,WB	Int J Mol Sci; Dietary Evodiamine Inhibits Atherosclerosis-Associated Changes in Vascular Smooth Muscle Cells; Authors - Yiwen Zha
human,mouse	WB,IF	Mol Cell Biochem; HRD1 reduction promotes cholesterol-induced vascular smooth muscle cell phenotypic change via endoplasmic reticulum stress; Authors - Linli Wang

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