Anticorps Polyclonal de lapin anti-MYH2-specific

MYH2-specific Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (4)

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications(5)

N° de cat : 55069-1-AP

Synonymes

Myosin 2, MYH2, Myosin heavy chain 2, MYHSA2, MyHC-IIa

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Galerie de données de validation

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  WB analysis of multi-cells/tissue using 55069-1-AP

  WB results of 55069-1-AP (anti-MYH2).

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  WB analysis using 55069-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 55069-1-AP (MYH2-specific antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of human skeletal muscle using 55069-1-AP

  human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 55069-1-AP (MYH2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human skeletal muscle using 55069-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 55069-1-AP (MYH2 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human skeletal muscle using 55069-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 55069-1-AP (MYH2 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human bladder using 55069-1-AP

  Immunohistochemical analysis of paraffin-embedded human bladder using 55069-1-AP (MYH2 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human bladder using 55069-1-AP

  Immunohistochemical analysis of paraffin-embedded human bladder using 55069-1-AP (MYH2 antibody) at dilution of 1:50 (under 40x lens).

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Applications testées

• Résultats positifs en WB: multi-cellules/tissus, tissu de muscle squelettique humain
• Résultats positifs en IHC: tissu de muscle squelettique humain, tissu vésical humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 3 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Informations sur le produit

55069-1-AP cible MYH2-specific dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: rat, canin, porc, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: myosin, heavy chain 2, skeletal muscle, adult
• Masse moléculaire calculée: 223 kDa
• Poids moléculaire observé: 200 kDa
• Numéro d’acquisition GenBank: NM_017534
• Symbole du gène: MYH2
• Identification du gène (NCBI): 4620
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

MYH2 (Myosin II) is a member of the class II or conventional myosin heavy chains. Myosin II was first isolated from muscle but is also found in non muscle cells, and it is especially enriched in highly motile cell types such as amoebae. It forms bipolar filaments that interact with actin filaments to produce contraction. And it is the motor protein that generates force to drive muscle contraction. Functions in skeletal muscle contraction. The antibody is specific to MYH2, will not bind other myosins. It is a skeletal muscle specific antibody.

Publications

Species	Application	Title
mouse	WB	Int J Mol Sci; Loss of Myostatin Alters Mitochondrial Oxidative Phosphorylation, TCA Cycle Activity, and ATP Production in Skeletal Muscle; Authors - Xueqiao Wang
pig	WB	BMC Genomics; Identification of different myofiber types in pigs muscles and construction of regulatory networks; Authors - Chenchen Li
canine	IHC	Animals (Basel); Comparative Analysis of Muscle Fibers in Selected Muscles of Working and Companion Dog Breeds; Authors - Cezary Osiak-Wicha
mouse	WB	J Muscle Res Cell Motil; The influences of ARHGEF9 on myoblasts migration and differentiation; Authors - Shuang Li
rat	IF	Sci Rep; Dietary supplementation with inulin improves burn-induced skeletal muscle atrophy by regulating gut microbiota disorders; Authors - Shan Gao

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