Anticorps Polyclonal de lapin anti-MYH3

MYH3 Polyclonal Antibody for WB, IP, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IHC, IF, IP, ELISA

Conjugaison

Non conjugué

Publications(15)

Avis (1)

N° de cat : 22287-1-AP

Synonymes

eMyHC, HEMHC, MYH3, MYHC EMB, MYHSE1, Myosin 3, Myosin heavy chain 3, SMHCE

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Galerie de données de validation

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  WB analysis of mouse embryo using 22287-1-AP

  mouse embryo tissue were subjected to SDS PAGE followed by western blot with 22287-1-AP (MYH3 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse skeletal muscle using 22287-1-AP

  IP result of anti-MYH3 (IP:22287-1-AP, 4ug; Detection:22287-1-AP 1:300) with mouse skeletal muscle tissue lysate 4000ug.

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  IHC staining of mouse heart using 22287-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 22287-1-AP (MYH3 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse heart using 22287-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 22287-1-AP (MYH3 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat heart using 22287-1-AP

  Immunohistochemical analysis of paraffin-embedded rat heart tissue slide using 22287-1-AP (MYH3 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat heart using 22287-1-AP

  Immunohistochemical analysis of paraffin-embedded rat heart tissue slide using 22287-1-AP (MYH3 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: tissu embryonnaire de souris
• Résultats positifs en IP: tissu de muscle squelettique de souris
• Résultats positifs en IHC: tissu cardiaque de rat, tissu cardiaque de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 11 publications below
• IF: See 6 publications below

Informations sur le produit

22287-1-AP cible MYH3 dans les applications de WB, IHC, IF, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, bovin, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: myosin, heavy chain 3, skeletal muscle, embryonic
• Masse moléculaire calculée: 234 kDa
• Poids moléculaire observé: 220-230 kDa
• Numéro d’acquisition GenBank: NM_002470
• Symbole du gène: MYH3
• Identification du gène (NCBI): 4621
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
mouse	WB	Nature; A non-canonical tricarboxylic acid cycle underlies cellular identity.; Authors - Paige K Arnold
rat	WB	Basic Res Cardiol; Alkaline nucleoplasm facilitates contractile gene expression in the mammalian heart.; Authors - Alzbeta Hulikova
mouse	IF	Cell Rep; Intramuscular adipose tissue restricts functional muscle recovery; Authors - Alessandra M Norris
mouse	WB	Int J Mol Sci; Molecular and Metabolic Mechanism of Low-Intensity Pulsed Ultrasound Improving Muscle Atrophy in Hindlimb Unloading Rats.; Authors - Lijun Sun
mouse	WB,IF	FASEB J; miR-206 family is important for mitochondrial and muscle function, but not essential for myogenesis in vitro.; Authors - Roza K Przanowska
		Am J Pathol; B4GALNT2 (GALGT2) Gene Therapy Reduces Skeletal Muscle Pathology in the FKRP P448L Mouse Model of Limb Girdle Muscular Dystrophy 2I.; Authors - Paul J Thomas

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Alessandra (Verified Customer) (03-23-2022)

Antigen retrieval was performed on Tibialis Anterior muscle cross sections. MYH3 in red (newly formed fibers); IgG in cyan (dying muscle fibers)

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:300
• Cell Tissue Type: Skeletal Muscle/Myoblast

MYH3 Antibody Immunofluorescence validation (1:300 dilution) in Skeletal Muscle/Myoblast (Cat no:22287-1-AP)

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