Anticorps Polyclonal de lapin anti-MYH4-Specific

MYH4-Specific Polyclonal Antibody for WB, IHC, IF-P, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (2)

Applications

WB, IHC, IF-P, ELISA

Conjugaison

Non conjugué

Publications(39)

N° de cat : 20140-1-AP

Synonymes

MYH2B, MYH4, MYH4-Specific, MyHC 2B, MyHC IIb, Myosin 4, Myosin heavy chain 2b, Myosin heavy chain 4, Myosin heavy chain IIb

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Galerie de données de validation

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  WB analysis of human skeletal muscle using 20140-1-AP

  human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 20140-1-AP (MYH4-Specific Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis using 20140-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20140-1-AP (MYH4-Specific antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of human skeletal muscle using 20140-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 20140-1-AP (MYH4-Specific Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human skeletal muscle using 20140-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 20140-1-AP (MYH4-Specific Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of mouse skeletal muscle using 20140-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 20140-1-AP (MYH4-Specific antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse skeletal muscle using 20140-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse skeletal muscle tissue using MYH4-Specific antibody (20140-1-AP) at dilution of 1:200, MYH2-specific antibody (55069-1-AP, green), MYH7-specific antibody (22280-1-AP, blue).

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Applications testées

• Résultats positifs en WB: tissu de muscle squelettique de souris, tissu de muscle squelettique de rat, tissu de muscle squelettique humain
• Résultats positifs en IHC: tissu de muscle squelettique humain, tissu de muscle squelettique de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF-P: tissu de muscle squelettique de souris,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 31 publications below
• IHC: See 2 publications below
• IF: See 10 publications below

Informations sur le produit

20140-1-AP cible MYH4-Specific dans les applications de WB, IHC, IF-P, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, canin, Humain, porc, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: myosin, heavy chain 4, skeletal muscle
• Masse moléculaire calculée: 223 kDa
• Poids moléculaire observé: 230 kDa
• Numéro d’acquisition GenBank: NM_017533
• Symbole du gène: MYH4
• Identification du gène (NCBI): 4622
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Myosin is one of the most important skeletal muscle proteins. MYH4 functions muscle contraction. The antibody is specific to MYH4.

Publications

Species	Application	Title
rat	IHC,IF	Sci Adv; Bioactive fiber-reinforced hydrogel to tailor cell microenvironment for structural and functional regeneration of myotendinous junction; Authors - Yuzhi Sun
mouse	IF	Nat Commun; The nuclear envelope protein Net39 is essential for muscle nuclear integrity and chromatin organization.; Authors - Andres Ramirez-Martinez
mouse	IF	Development; The long noncoding RNA Meg3 regulates myoblast plasticity and muscle regeneration through epithelial-mesenchymal transition; Authors - Tiffany L. Dill
mouse	IF	Am J Pathol; Klotho null mutation involvement in adenosine A2B receptor-related skeletal muscle degeneration; Authors - Hung-Liang Pai
mouse	IF	Development; The long noncoding RNA Meg3 regulates myoblast plasticity and muscle regeneration through epithelial-mesenchymal transition.; Authors - Tiffany L Dill
mouse	WB	Int J Mol Sci; Loss of Myostatin Alters Mitochondrial Oxidative Phosphorylation, TCA Cycle Activity, and ATP Production in Skeletal Muscle; Authors - Xueqiao Wang

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