Anticorps Polyclonal de lapin anti-MYH9

• Phare
• Validé par KD/KO

MYH9 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, IF, FC, CoIP, ELISA

Conjugaison

Non conjugué

Publications

29

N° de cat : 11128-1-AP

Synonymes

MYH9, Myosin 9, Myosin heavy chain 9, myosin IIa, Nonmuscle myosin IIa

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Galerie de données de validation

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  WB analysis using 11128-1-AP

  Various cell lysates were subjected to SDS PAGE followed by western blot with 11128-1-AP (MYH9 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 11128-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 11128-1-AP (MYH9 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse kidney using 11128-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 11128-1-AP (MYH9 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse colon using 11128-1-AP

  mouse colon tissue were subjected to SDS PAGE followed by western blot with 11128-1-AP (MYH9 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 11128-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 11128-1-AP (MYH9 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 11128-1-AP

  IP Result of anti-MYH9 (IP:11128-1-AP, 5ug; Detection:11128-1-AP 1:1000) with mouse brain tissue lysate 4000ug.

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  IHC staining of human lung cancer using 11128-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11128-1-AP (MYH9 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 11128-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11128-1-AP (MYH9 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse kidney using 11128-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 11128-1-AP (MYH9 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse kidney using 11128-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 11128-1-AP (MYH9 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 11128-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 11128-1-AP (MYH9 antibody) at dilution of 1:50 (under 10x lens).

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  IF Staining of mouse small intestine using 11128-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse small intestine tissue using MYH9 antibody (11128-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of HepG2 using 11128-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using MYH9 antibody (11128-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of HepG2 using 11128-1-AP

  Immunofluorescent analysis of HepG2 cells, using MYH9 antibody 11128-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

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  FC experiment of HepG2 using 11128-1-AP

  1X10^6 HepG2 cells were intracellularly stained with 0.2 ug Anti-Human MYH9 (11128-1-AP) and Fluorescein (FITC)-conjugated Affinipure Goat Anti-Rabbit IgG(H+L) at dilution 1:200 (red), or 0.2 ug Control Antibody. Cells were fixed with 90% MeOH .

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Applications testées

• Résultats positifs en WB: cellules A549, cellules U2OS, tissu cérébral de souris, tissu cérébral humain, tissu de côlon de souris, tissu rénal de souris
• Résultats positifs en IP: tissu cérébral de souris
• Résultats positifs en IHC: tissu de cancer du poumon humain, tissu rénal de souris, tissu rénal humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: tissu d'intestin grêle de souris, cellules HepG2
• Résultats positifs en cytométrie: cellules HepG2

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 22 publications below
• IHC: See 7 publications below
• IF: See 16 publications below
• IP: See 3 publications below
• CoIP: See 3 publications below

Informations sur le produit

11128-1-AP cible MYH9 dans les applications de WB, IP, IHC, IF, FC, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: MYH9 Protéine recombinante Ag1612
• Nom complet: myosin, heavy chain 9, non-muscle
• Masse moléculaire calculée: 227 kDa
• Poids moléculaire observé: 224 kDa
• Numéro d’acquisition GenBank: BC011915
• Symbole du gène: MYH9
• Identification du gène (NCBI): 4627
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

MYH9 (Myosin-9) appears to play a role in cytokinesis, cell shape, and specialized functions such as secretion and capping. MYH9 is a hexameric protein that consists of two heavy chains (~200 kDa) and two pairs of non-identical light chains (15-22 kDa), a pair of essential or alkali light chains, and a pair of regulatory light chains (PMID: 12800156).

Publications

Species	Application	Title
human	WB,IF	Acta Pharm Sin B; Ginsenoside Rg1 ameliorates blood-brain barrier disruption and traumatic brain injury via attenuating macrophages derived exosomes miR-21 release.; Authors - Kefeng Zhai
human	WB	J Exp Clin Cancer Res; ACTN1 promotes HNSCC tumorigenesis and cisplatin resistance by enhancing MYH9-dependent degradation of GSK-3β and integrin β1-mediated phosphorylation of FAK; Authors - Li Cui
human	WB,IF	Cell Death Differ; Glycoprotein PTGDS promotes tumorigenesis of diffuse large B-cell lymphoma by MYH9-mediated regulation of Wnt-β-catenin-STAT3 signaling; Authors - Shunfeng Hu ; KD Validated
human,mouse	WB,IHC,IF	Cell Rep; Mechanotransduction in response to ECM stiffening impairs cGAS immune signaling in tumor cells; Authors - Yingqi Liu
mouse	IF,CoIP,WB	Theranostics; Salvia miltiorrhiza-derived miRNAs suppress vascular remodeling through regulating OTUD7B/KLF4/NMHC IIA axis.; Authors - Gao-Shan Yang
mouse	IF,WB	Stem Cell Reports; Non-muscle myosin heavy chain 9 maintains intestinal homeostasis by preventing epithelium necroptosis and colitis adenoma formation.; Authors - Shan Wang ; KO Validated

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