Anticorps Polyclonal de lapin anti-MYL12A

MYL12A Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (2)

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications(5)

N° de cat : 16287-1-AP

Synonymes

Epididymis secretory protein Li 24, HEL-S-24, MLC 2B, MLC-2B, MLCB

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Galerie de données de validation

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  WB analysis of NIH/3T3 using 16287-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 16287-1-AP (MYL12A Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 16287-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 16287-1-AP (MYL12A antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 16287-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 16287-1-AP (MYL12A antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 16287-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 16287-1-AP (MYL12A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human colon cancer using 16287-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 16287-1-AP (MYL12A antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 16287-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 16287-1-AP (MYL12A antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules NIH/3T3, cellules HeLa, cellules Jurkat
• Résultats positifs en IHC: tissu de cancer du côlon humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:2000-1:8000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 3 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Informations sur le produit

16287-1-AP cible MYL12A dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, porc, souris, Grenouille
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: MYL12A Protéine recombinante Ag9413
• Nom complet: myosin, light chain 12A, regulatory, non-sarcomeric
• Masse moléculaire calculée: 171 aa, 20 kDa
• Poids moléculaire observé: 19-20 kDa
• Numéro d’acquisition GenBank: BC016372
• Symbole du gène: MYL12A
• Identification du gène (NCBI): 10627
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Myosin II is an actin-binding protein composed of MHC (myosin heavy chain), MLCs/MRLCs (myosin regulatory light chains) and ELCs (essential light chains). The Myosin Light Chains are divided into basic MLC (MLC I) and regulatory MLC (MLC II or Myosin Regulatory Light Chain, MRLC). MRLC can be classified into two major types: : class I MRLC found in muscles (MLC1, MLC2, MLC3, MYL4, MYL5, MYL6, MYL7, etc), and class II MRLCs present in non-muscle cells (such as MYL9, MYL12A, MYL12B).

Publications

Species	Application	Title
pig	WB	Biochem J; Kinase-related protein/telokin inhibits Ca2+-independent contraction in Triton-skinned guinea pig taenia coli.; Authors - Shcherbakova Olga V OV
mouse	WB	Front Physiol; Distinct Roles of Smooth Muscle and Non-muscle Myosin Light Chain-Mediated Smooth Muscle Contraction.; Authors - Jie Sun
human	IHC	Biofizika; [Novel peptide inhibitors of the myosin light chain kinase suppress hyperpermeability of vascular endothelium].; Authors - Marchenko A V AV
mouse	WB	EMBO J; Salmonella manipulates macrophage migration via SteC-mediated myosin light chain activation to penetrate the gut-vascular barrier; Authors - Yuanji Dai
Frog	IF	J Assoc Res Otolaryngol; Influence of Myosin Regulatory Light Chain and Myosin Light Chain Kinase on the Physiological Function of Inner Ear Hair Cells; Authors - Ryohei Oya

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