Anticorps Monoclonal anti-MYL3

MYL3 Monoclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, porc, rat, souris

Applications

WB, IHC, RIP, ELISA

Conjugaison

Non conjugué

Publications(3)

CloneNo.

2E4B2

N° de cat : 66286-1-Ig

Synonymes

Cardiac myosin light chain 1, CMH8, CMLC1, MLC1SB, MLC1V, MYL3, Myosin light chain 3, VLC1

---

Galerie de données de validation

• 

  WB analysis using 66286-1-Ig

  Various tissue lysates were subjected to SDS PAGE followed by western blot with 66286-1-Ig (MYL3 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human skeletal muscle using 66286-1-Ig

  human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 66286-1-Ig (MYL3 Antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 66286-1-Ig

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 66286-1-Ig (MYL3 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human heart using 66286-1-Ig

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 66286-1-Ig (MYL3 Antibody) at dilution of 1:600 (under 10x lens).

• 

  IHC staining of human heart using 66286-1-Ig

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 66286-1-Ig (MYL3 Antibody) at dilution of 1:600 (under 40x lens).

• 

  IHC staining of human skeletal muscle using 66286-1-Ig

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 66286-1-Ig (MYL3 Antibody) at dilution of 1:600 (under 10x lens).

• 

  IHC staining of human skeletal muscle using 66286-1-Ig

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 66286-1-Ig (MYL3 Antibody) at dilution of 1:600 (under 40x lens).

---

Applications testées

• Résultats positifs en WB: tissu de muscle squelettique humain, tissu cardiaque de porc, tissu cardiaque de rat, tissu cardiaque de souris, tissu cardiaque humain
• Résultats positifs en IHC: tissu cardiaque humain, tissu de muscle squelettique humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochimie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 3 publications below
• RIP: See 1 publications below

Informations sur le produit

66286-1-Ig cible MYL3 dans les applications de WB, IHC, RIP, ELISA et montre une réactivité avec des échantillons Humain, porc, rat, souris

• Réactivité: Humain, porc, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: MYL3 Protéine recombinante Ag24535
• Nom complet: myosin, light chain 3, alkali; ventricular, skeletal, slow
• Masse moléculaire calculée: 22 kDa
• Poids moléculaire observé: 22-25 kDa
• Numéro d’acquisition GenBank: BC009790
• Symbole du gène: MYL3
• Identification du gène (NCBI): 4634
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

MYL3, also named as MLC1v, is an essential light chain of myosin that is associated with muscle contraction. It is expressed in ventricular and slow skeletal muscle. MYL3 may serve as a target for caspase-3 in dying cardiomyocytes. Mutations of MYL3 gene cause hypertrophic cardiomyopathy. MYL3 has been identified as potential serum biomarker for drug induced myotoxicity. Great increase in MYL3 serum concentration has been observed in rats with cardiac and skeletal muscle injury. (PMID:21685905)

Publications

Species	Application	Title
human	WB	Antioxidants (Basel); Ganoderma lucidum Spore Powder Alleviates Metabolic-Associated Fatty Liver Disease by Improving Lipid Accumulation and Oxidative Stress via Autophagy; Authors - Yuxuan Zhang
mouse	WB,RIP	Research (Wash D C); Circ-0001283 Aggravates Cardiac Hypertrophy by Targeting Myosin Light Chain 3 Protein; Authors - Wenjing Wang
human	WB	Transl Oncol; ACAT1 suppresses clear cell renal cell carcinoma progression by AMPK mediated fatty acid metabolism; Authors - Ming Zheng

{{ptg:RelatedPrimaryAntibodies}}