Anticorps Monoclonal anti-MYL4

• Phare
• Validé par KD/KO

MYL4 Monoclonal Antibody for WB, IHC, IF-P, FC (Intra), ELISA

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain, porc, rat, souris

Applications

WB, IHC, IF-P, FC (Intra), ELISA

Conjugaison

Non conjugué

Publications(6)

CloneNo.

2F5C1

N° de cat : 67533-1-Ig

Synonymes

2F5C1, MLC1, MYL 4, Myosin light chain 4, Myosin light chain alkali GT-1 isoform

---

Galerie de données de validation

• 

  WB analysis using 67533-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67533-1-Ig (MYL4 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 67533-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67533-1-Ig (MYL4 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human heart using 67533-1-Ig

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 67533-1-Ig (MYL4 antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse heart using 67533-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using MYL4 antibody (67533-1-Ig, Clone: 2F5C1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  FC experiment of C2C12 using 67533-1-Ig

  1X10^6 C2C12 cells were intracellularly stained with 0.4 ug Anti-Human MYL4 (67533-1-Ig, Clone:2F5C1) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

---

Applications testées

• Résultats positifs en WB: tissu cardiaque de porc, cellules K-562, cellules TF-1, tissus cardiaques de rat, tissus cardiaques de souris
• Résultats positifs en IHC: tissu cardiaque humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF-P: tissu cardiaque de souris,
• Résultats positifs en FC (Intra): cellules C2C12,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochimie (IHC): IHC : 1:150-1:600
• Immunofluorescence (IF)-P: IF-P : 1:200-1:800
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 4 publications below
• IF: See 3 publications below

Informations sur le produit

67533-1-Ig cible MYL4 dans les applications de WB, IHC, IF-P, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain, porc, rat, souris

• Réactivité: Humain, porc, rat, souris
• Réactivité citée: rat, Humain, porc, souris
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: MYL4 Protéine recombinante Ag19244
• Nom complet: myosin, light chain 4, alkali; atrial, embryonic
• Masse moléculaire calculée: 197 aa, 22 kDa
• Poids moléculaire observé: 22-25 kDa
• Numéro d’acquisition GenBank: BC030228
• Symbole du gène: MYL4
• Identification du gène (NCBI): 4635
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Myosin light‐chain 4 (MYL4) encodes an essential myosin light chain that is expressed in embryonic whole heart and skeletal muscle. It is specifically expressed in human atria after birth. MYL4 is essential for atrial electrical, functional, and structural integrity, and MYL4 dysfunction can produce severe inherited atrial cardiomyopathy.

Publications

Species	Application	Title
human	IF	Front Cell Dev Biol; HiPS-Endothelial Cells Acquire Cardiac Endothelial Phenotype in Co-culture With hiPS-Cardiomyocytes.; Authors - Emmi Helle
rat	WB	J Proteome Res; Data-Independent Acquisition Proteomics and N-Terminomics Methods Reveal Alterations in Mitochondrial Function and Metabolism in Ischemic-Reperfused Hearts; Authors - Bridgette Hartley
mouse	WB,IF	Genes (Basel); Molecular Mechanism of MYL4 Regulation of Skeletal Muscle Development in Pigs; Authors - Xueli Xu ; KD Validated
pig	WB	Gene; Haplotypes within the regulatory region of MYL4 are associated with pig muscle fiber size; Authors - Shixiong Dong
mouse	WB	Cell Biosci; Motoneurons innervation determines the distinct gene expressions in multinucleated myofibers; Authors - Lei Bai
mouse	IF	Front Med; Distinct mononuclear diploid cardiac subpopulation with minimal cell-cell communications persists in embryonic and adult mammalian heart; Authors - Miaomiao Zhu

{{ptg:RelatedPrimaryAntibodies}}