Anticorps Monoclonal anti-Myocilin

Myocilin Monoclonal Antibody for IHC, WB,ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain, porc, rat, souris

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

6

CloneNo.

4B1F6

N° de cat : 60357-1-Ig

Synonymes

GLC1A, GPOA, JOAG, JOAG1, MYOC, myocilin, TIGR

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Galerie de données de validation

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  WB analysis of pig heart using 60357-1-Ig

  pig heart tissue were subjected to SDS PAGE followed by western blot with 60357-1-Ig (Myocilin antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat heart using 60357-1-Ig

  rat heart tissue were subjected to SDS PAGE followed by western blot with 60357-1-Ig (Myocilin antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human skeletal muscle using 60357-1-Ig

  human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 60357-1-Ig (Myocilin antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human spleen using 60357-1-Ig

  human spleen tissue were subjected to SDS PAGE followed by western blot with 60357-1-Ig (Myocilin antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human heart using 60357-1-Ig

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 60357-1-Ig (Myocilin antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human heart using 60357-1-Ig

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 60357-1-Ig (Myocilin antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human skeletal muscle using 60357-1-Ig

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 60357-1-Ig (Myocilin antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human skeletal muscle using 60357-1-Ig

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 60357-1-Ig (Myocilin antibody) at dilution of 1:50 (under 40x lens).

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Applications testées

• Résultats positifs en WB: tissu cardiaque de porc, tissu cardiaque de rat, tissu de muscle squelettique humain, tissu splénique humain
• Résultats positifs en IHC: tissu cardiaque humain, tissu de muscle squelettique humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:8000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 4 publications below
• IHC: See 1 publications below
• IF: See 2 publications below

Informations sur le produit

60357-1-Ig cible Myocilin dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, porc, rat, souris

• Réactivité: Humain, porc, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Myocilin Protéine recombinante Ag5492
• Nom complet: myocilin, trabecular meshwork inducible glucocorticoid response
• Masse moléculaire calculée: 57 kDa
• Poids moléculaire observé: 50-57 kDa
• Numéro d’acquisition GenBank: BC029261
• Symbole du gène: MYOC
• Identification du gène (NCBI): 4653
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Myocilin (MYOC), also known as TIGR or GLC1A, is a secreted glycoprotein of the olfactomedin family, originally identified in trabecular meshwork cells after prolonged treatment with glucocorticoids, and, independently, in the retina (PMID: 9176893; 9169133). It may participate in the obstruction of fluid outflow in the trabecular meshwork. Defects in MYOC are the cause of primary open angle glaucoma (POAG) (PMID: 21709622).

Publications

Species	Application	Title
	IHC	Stem Cell Res Ther; Human amniotic epithelial cell transplantation improves scar remodeling in a rabbit model of acute vocal fold injury: a pilot study.; Authors - Yourka D Tchoukalova
rat	WB	Exp Eye Res; Sympathetic activation leads to Schlemm's canal expansion via increasing vasoactive intestinal polypeptide secretion from trabecular meshwork; Authors - Dingwen Xu
human	WB,IF	Int Ophthalmol; The effect of ET1-CTGF mediated pathway on the accumulation of extracellular matrix in the trabecular meshwork and its contribution to the increase in IOP; Authors - Junming Wang
human	WB	Transl Vis Sci Technol; Inhibition of TGF-β2-Induced Trabecular Meshwork Fibrosis by Pirfenidone; Authors - Xiaofeng Zhu
mouse	IF	Res Sq; Lentiviral mediated delivery of CRISPR/Cas9 reduces intraocular pressure in a mouse model of myocilin glaucoma; Authors - Shruti V Patil
human	WB	Biochem Biophys Res Commun; Identification and application of p75 neurotrophin receptor-expressing human trabecular meshwork progenitor cells.; Authors - Susumu Hara