Anticorps Recombinant de lapin anti-MYOF

MYOF Recombinant Antibody for FC (Intra), ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

FC (Intra), ELISA

Conjugaison

Non conjugué

CloneNo.

230213C6

N° de cat : 82917-1-RR

Synonymes

Myoferlin, KIAA1207, Fer-1-like protein 3, FER1L3, Fer 1 like protein 3

---

Galerie de données de validation

• 

  FC experiment of HepG2 using 82917-1-RR

  1x10^6 HepG2 cells were intracellularly stained with 0.25 ug Anti-Human MYOF (82917-1-RR, Clone:230213C6) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:166 (red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP, Clone: ) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

• 

  FC experiment of HeLa using 82917-1-RR

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug Anti-Human MYOF (82917-1-RR, Clone:230213C6) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:166 (red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP, Clone: ) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

---

Applications testées

• Résultats positifs en FC (Intra): cellules HepG2, cellules HeLa

Dilution recommandée

• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

82917-1-RR cible MYOF dans les applications de FC (Intra), ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: myoferlin
• Masse moléculaire calculée: 235 kDa
• Numéro d’acquisition GenBank: NM_133337
• Symbole du gène: MYOF
• Identification du gène (NCBI): 26509
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

MYOF, also named FER1L3 and KIAA1207, belongs to the Ferlin family. MYOF is a calcium/phospholipid-binding protein that plays a role in the plasmalemma repair mechanism of endothelial cells that permits rapid resealing of membranes disrupted by mechanical stress. It is involved in endocytic recycling. MYOF is implicated in VEGF signal transduction by regulating the levels of the receptor KDR.

{{ptg:RelatedPrimaryAntibodies}}