Anticorps Polyclonal de lapin anti-Myozenin 2

Myozenin 2 Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications(2)

N° de cat : 11450-1-AP

Synonymes

C4orf5, Calsarcin-1, FATZ related protein 2, MYOZ2, Myozenin 2

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Galerie de données de validation

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  WB analysis using 11450-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11450-1-AP (Myozenin 2 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  IHC staining of human heart using 11450-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 11450-1-AP (Myozenin 2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human heart using 11450-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 11450-1-AP (Myozenin 2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 11450-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 11450-1-AP (Myozenin 2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 11450-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 11450-1-AP (Myozenin 2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: tissu cardiaque de souris, tissu cardiaque de rat
• Résultats positifs en IHC: tissu cardiaque humain, tissu cérébral de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:12000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below

Informations sur le produit

11450-1-AP cible Myozenin 2 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Myozenin 2 Protéine recombinante Ag2008
• Nom complet: myozenin 2
• Masse moléculaire calculée: 30 kDa
• Poids moléculaire observé: 30-35 kDa
• Numéro d’acquisition GenBank: BC017402
• Symbole du gène: Myozenin 2
• Identification du gène (NCBI): 51778
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

MYOZ2 gene encodes for the calcineurin-binding protein myozenin-2 (or Calsarcin-1) belonging to myozenin family. Defects in MYOZ2 gene are the cause of familial hypertrophic cardiomyopathy type 16 (CMH16), a hereditary heart disorder characterized by ventricular hypertrophy. The disorder has inter- and intrafamilial variability ranging from benign to malignant forms with high risk of cardiac failure and sudden cardiac death (PMID: 17347475). Myozenins may serve as intracellular binding proteins involved in linking Z line proteins such as alpha-actinin, and gamma-filamin. Important role in the modulation of calcineurin signaling and myofibrillogenesis of myozenin-2 have been elucidated (PMID: 11114196). Proteintech's Myozenin-2 antibody 11450-1-AP was raised against full-length protein of human origin and is able to detect band of approximate 34 kDa in Western blotting.

Publications

Species	Application	Title
mouse	WB	Sci Rep; G9a inhibits MEF2C activity to control sarcomere assembly.; Authors - Jin Rong Ow
rat	WB	J Pain Res; Quantitative proteomics analysis to identify biomarkers of chronic myofascial pain and therapeutic targets of dry needling in a rat model of myofascial trigger points.; Authors - Li-Hui Li

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