Anticorps Recombinant de lapin anti-Methylmalonyl Coenzyme A mutase/MUT

Methylmalonyl Coenzyme A mutase/MUT Recombinant Antibody for WB, IHC, FC (Intra), ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, FC (Intra), ELISA

Conjugaison

Non conjugué

CloneNo.

242198F11

N° de cat : 84879-5-RR

Synonymes

MUT, 242198F11, EC:5.4.99.2, MCM, Methylmalonyl CoA isomerase

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Galerie de données de validation

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  WB analysis using 84879-5-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84879-5-RR (Methylmalonyl Coenzyme A mutase/MUT antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of human prostate cancer using 84879-5-RR

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 84879-5-RR (Methylmalonyl Coenzyme A mutase/MUT antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human prostate cancer using 84879-5-RR

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 84879-5-RR (Methylmalonyl Coenzyme A mutase/MUT antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  FC experiment of A431 using 84879-5-RR

  1x10^6 A431 cells were intracellularly stained with 0.25 ug Methylmalonyl Coenzyme A mutase/MUT Recombinant antibody (84879-5-RR, Clone:242198F11) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  Affinity and Kinetic Characterization of 84879-5-RR

  Biolayer interferometry (BLl) kinetic assays of 84879-5-RR against Human Methylmalonyl Coenzyme A mutase/MUT were performed. The affinity constant is 1.83 nM.

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules HeLa, cellules HepG2, cellules K-562, tissu cérébral de souris, tissu hépatique de rat
• Résultats positifs en IHC: tissu de cancer de la prostate humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en FC (Intra): cellules A431,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochimie (IHC): IHC : 1:200-1:800
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

84879-5-RR cible Methylmalonyl Coenzyme A mutase/MUT dans les applications de WB, IHC, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Methylmalonyl Coenzyme A mutase/MUT Protéine recombinante Ag10523
• Nom complet: methylmalonyl Coenzyme A mutase
• Masse moléculaire calculée: 750 aa, 83 kDa
• Poids moléculaire observé: 78 kDa
• Numéro d’acquisition GenBank: BC016282
• Symbole du gène: MUT
• Identification du gène (NCBI): 4594
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Methylmalonyl Coenzyme A mutase (MUT) is an enzyme that plays a crucial role in the metabolism of certain amino acids and fatty acids. Mutations in the MUT gene can lead to methylmalonic acidemia, a metabolic disorder characterized by the accumulation of toxic compounds such as methylmalonyl-CoA and propionyl-CoA. This condition can cause severe health issues including developmental delays, metabolic acidosis, and neurological problems. MUT is essential for maintaining normal metabolic processes and its dysfunction can have significant health implications, highlighting its importance in both basic metabolism and clinical medicine.

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