Anticorps Recombinant de lapin anti-Midkine

Midkine Recombinant Antibody for IHC, IF/ICC, FC (Intra), ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

IHC, IF/ICC, FC (Intra), ELISA

Conjugaison

Non conjugué

CloneNo.

241880H1

N° de cat : 84815-1-RR

Synonymes

MDK, 241880H1, Amphiregulin-associated protein, Neurite outgrowth-promoting factor 2, Neurite outgrowth-promoting protein

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Galerie de données de validation

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  IHC staining of mouse embryo using 84815-1-RR

  Immunohistochemical analysis of paraffin-embedded mouse embryo tissue slide using 84815-1-RR (Midkine antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse embryo using 84815-1-RR

  Immunohistochemical analysis of paraffin-embedded mouse embryo tissue slide using 84815-1-RR (Midkine antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 84815-1-RR

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using Midkine antibody (84815-1-RR, Clone: 241880H1 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  FC experiment of HeLa using 84815-1-RR

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug Midkine Recombinant antibody (84815-1-RR, Clone:241880H1) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  Affinity and Kinetic Characterization of 84815-1-RR

  Biolayer interferometry (BLl) kinetic assays of 84815-1-RR against Human Midkine were performed. The affinity constant is 35.9 nM.

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Applications testées

• Résultats positifs en IHC: tissu embryonnaire de souris, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HeLa,
• Résultats positifs en FC (Intra): cellules HeLa,

Dilution recommandée

• Immunohistochimie (IHC): IHC : 1:200-1:800
• Immunofluorescence (IF)/ICC: IF/ICC : 1:500-1:2000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

84815-1-RR cible Midkine dans les applications de IHC, IF/ICC, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Midkine Protéine recombinante Ag24547
• Nom complet: midkine (neurite growth-promoting factor 2)
• Masse moléculaire calculée: 16 kDa
• Numéro d’acquisition GenBank: BC011704
• Symbole du gène: Midkine
• Identification du gène (NCBI): 4192
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Midkine is a heparin-binding growth factor identified over 20 years ago and enhances the survival, migration and many other activities of target cells. Midkine is rich in both basic amino acids and cysteine, and is not related to most other growth factors/cytokines. It is strongly expressed during embryonic periods, especially at the midgestation stage, and plays important roles in development, especially in neurogenesis. Midkine expression in adult tissue is generally weak or undetectable, and it is induced upon injury and exerts many activities related to tissue repair. The biological activities of midkine in malignant tumors include proliferation, angiogenesis, invasion and metastasis. Various cancers express significantly higher levels of the midkine protein in early stage tumor tissues than in adjacent normal tissue.

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