Anticorps Recombinant de lapin anti-MMP9

MMP9 Recombinant Antibody for FC (Intra)

Hôte / Isotype

Lapin / IgG

Réactivité testée

souris

Applications

FC (Intra)

Conjugaison

Non conjugué

CloneNo.

230069F1

N° de cat : 98396-1-RR

Synonymes

230069F1, AW743869, B/MMP9, Clg4b, matrix metallopeptidase 9

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Galerie de données de validation

• 

  FC experiment of RAW 264.7 using 98396-1-RR

  1x10^6 untreated or LPS and Brefeldin A treated RAW 264.7 cells were intracellularly stained with 0.13 ug Anti-Mouse Mmp9 Rabbit RecAb (98396-1-RR, Clone: 230069F1) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  Affinity and Kinetic Characterization of 98396-1-RR

  Biolayer interferometry (BLl) kinetic assays of 98396-1-RR against Mouse MMP9 were performed. The affinity constant is below 1 pM.

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Applications testées

• Résultats positifs en FC (Intra): cellules RAW 264.7 traitées au LPS et à la bréfeldine A,

Dilution recommandée

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

98396-1-RR cible MMP9 dans les applications de FC (Intra) et montre une réactivité avec des échantillons souris

• Réactivité: souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: MMP9 Protéine recombinante Eg0452
• Nom complet: matrix metallopeptidase 9
• Masse moléculaire calculée: 81kd
• Numéro d’acquisition GenBank: NM_013599
• Symbole du gène: Mmp9
• Identification du gène (NCBI): 17395
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS with 0.09% sodium azide
• Conditions de stockage: Store at 2 - 8°C. Stable for one year after shipment.

Informations générales

MMP9 (matrix metallopeptidase 9), also named as Gelatinase B, is a member of matrix metalloproteinase (MMP) family. The MMP family of enzymes is comprised of critically important extracellular matrix remodeling proteases whose activity has been implicated in normal embryogenesis, tissue remodelling and many diseases such as arthritis, cancer, periodontitis, glomerulonephritis, encephalomyelitis, atherosclerosis and tissue ulceration. MMP9 is produced by a variety of normal and transformed cells including neutrophils, monocytes, macrophages, astrocytes, fbroblasts, osteoclasts and so on. Transgenic mouse models report that MMP9 contributes to skin carcinogenesis, suppresses development of experimental abdominal aortic aneurysms, and triggers the angiogenic switch during carcinogenesis.

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