Anticorps Monoclonal anti-Mouse IgG1 isotype control

Mouse IgG1 isotype control Monoclonal Antibody for FC, WB, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Megathura crenulata et plus (1)

Applications

WB, RIP, IP, IHC, FC, CoIP, Cell treatment, ELISA

Conjugaison

Non conjugué

Publications

8

CloneNo.

1F8D3

N° de cat : 66360-1-Ig

Synonymes

Kappa light chain, Mouse IgG1, Mouse IgG1 isotype control

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• Galerie de données de validation
• Proteintech vs. Other Vendors

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  WB analysis using 66360-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66360-1-Ig (Mouse IgG1 isotype control antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis using 66360-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66360-1-Ig (Mouse IgG1 isotype control antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  FC experiment of K-562 using 66360-1-Ig

  1X10^6 K-562 cells were stained with 0.20ug Mouse IgG1 isotype control antibody (66360-1-Ig, red) and another control antibody (blue). Fixed with 90% MeOH.

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Applications testées

• Résultats positifs en WB: ,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Flow Cytometry (FC): FC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below
• IHC: See 1 publications below
• IP: See 2 publications below
• CoIP: See 1 publications below
• RIP: See 1 publications below

Informations sur le produit

66360-1-Ig cible Mouse IgG1 isotype control dans les applications de WB, RIP, IP, IHC, FC, CoIP, Cell treatment, ELISA et montre une réactivité avec des échantillons Megathura crenulata

• Réactivité: Megathura crenulata
• Réactivité citée: souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: Mouse IgG1 isotype control
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

This antibody is used as the isotype control of mouse IgG1 (Kappa). It can be used as the isotype control in Flow Cytometry, Immuno-Precipitation and other experiments. The immunogen of this antibody is Keyhole Limpet Hemocyanin (KLH).

Publications

Species	Application	Title
	CoIP	FASEB J; Bile acid restrained T cell activation explains cholestasis aggravated hepatitis B virus infection; Authors - Chujie Ding
	IP	Exp Cell Res; CBL aggravates Ang II-induced cardiac hypertrophy via the VHL/HIF-1α pathway.; Authors - Yuting Yang
	IHC	Am J Rhinol Allergy; Co-Expression and Localization of Angiotensin-Converting Enzyme-2 (ACE2) and the Transmembrane Serine Protease 2 (TMPRSS2) in Paranasal Ciliated Epithelium of Patients with Chronic Rhinosinusitis.; Authors - Tomohiro Kawasumi
	RIP	Cell Biosci; PABPN1 regulates mRNA alternative polyadenylation to inhibit bladder cancer progression; Authors - Liang Chen
		Stem Cell Res Ther; Menstrual blood-derived endometrial stem cells alleviate neuroinflammation by modulating M1/M2 polarization in cell and rat Parkinson's disease models; Authors - Han Li
	IP	Acta Biochim Biophys Sin (Shanghai); PGAM5 deacetylation mediated by SIRT2 facilitates lipid metabolism and liver cancer proliferation; Authors - Gongyu Fu