Anticorps Monoclonal anti-Mouse IgG2a isotype control

Mouse IgG2a isotype control Monoclonal Antibody for FC, WB, ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Megathura crenulata

Applications

WB, IP, FC, ELISA

Conjugaison

Non conjugué

Publications

2

CloneNo.

11A1B2

Avis (1)

N° de cat : 66360-2-Ig

Synonymes

Mouse IgG2a, Mouse IgG2a isotype control

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• Galerie de données de validation
• Proteintech vs. Other Vendors

• 

  WB analysis using 66360-2-Ig

  Various lysates (50 μg/lane) were subjected to SDS PAGE followed by western blot with 66360-2-Ig (Mouse IgG2a isotype control antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  FC experiment of HepG2 using 66360-2-Ig

  1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Mouse IgG2a isotype control (66360-2-Ig, Clone: 11A1B2) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Cytochrome c Mouse McAb (66264-1-Ig, Clone: 2D8D11) (green) or unstained (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

Résultats positifs en WB
Résultats positifs en cytométrie

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Flow Cytometry (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• IP: See 1 publications below
• FC: See 1 publications below

Informations sur le produit

66360-2-Ig cible Mouse IgG2a isotype control dans les applications de WB, IP, FC, ELISA et montre une réactivité avec des échantillons Megathura crenulata

• Réactivité: Megathura crenulata
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Protéine recombinante
• Nom complet: Mouse IgG2a isotype control
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

This antibody is used as the isotype control of mouse IgG2a. It can be used as the isotype control in Flow Cytometry, Immuno-Precipitation and other experiments. The immunogen of this antibody is Keyhole Limpet Hemocyanin (KLH).

Publications

Species	Application	Title
	FC	Biosens Bioelectron; Spherical nucleic acids-based cascade signal amplification for highly sensitive detection of exosomes.; Authors - Lei Wang
	IP	Nature; CDK11 regulates pre-mRNA splicing by phosphorylation of SF3B1; Authors - Milan Hluchý

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Niklas (Verified Customer) (07-31-2023)

better staining control than secondary only control

• Applications: Immunofluorescence, Flow Cytometry
• Primary Antibody Dilution: 1:200-1:400
• Cell Tissue Type: mouse and human heart