Anticorps Polyclonal de lapin anti-MSI2

MSI2 Polyclonal Antibody for WB, IHC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, IP, RIP, ELISA

Conjugaison

Non conjugué

Publications(9)

N° de cat : 10770-1-AP

Synonymes

Musashi-2, RNA-binding protein Musashi homolog 2, Musashi 2, MSI2H

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Galerie de données de validation

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  WB analysis of PC-12 using 10770-1-AP

  PC-12 cells were subjected to SDS PAGE followed by western blot with 10770-1-AP (MSI2 antibody at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 10770-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 10770-1-AP (MSI2 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of COLO 320 using 10770-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 10770-1-AP (MSI2 antibody at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of C6 using 10770-1-AP

  C6 cells were subjected to SDS PAGE followed by western blot with 10770-1-AP (MSI2 antibody at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of C6 using 10770-1-AP

  C6 cells were subjected to SDS PAGE followed by western blot with 10770-1-AP (MSI2 antibody at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 10770-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 10770-1-AP (MSI2 antibody at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-12 using 10770-1-AP

  PC-12 cells were subjected to SDS PAGE followed by western blot with 10770-1-AP (MSI2 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 10770-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 10770-1-AP (MSI2 antibody at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-12 using 10770-1-AP

  PC-12 cells were subjected to SDS PAGE followed by western blot with 10770-1-AP (MSI2 antibody at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of MCF-7 using 10770-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 10770-1-AP (MSI2 antibody) at dilution of 1:500.

• 

  IHC staining of human ovary tumor using 10770-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 10770-1-AP (MSI2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary tumor using 10770-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 10770-1-AP (MSI2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse liver using 10770-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 10770-1-AP (MSI2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 10770-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10770-1-AP (MSI2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse liver using 10770-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 10770-1-AP (MSI2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 10770-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10770-1-AP (MSI2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules PC-12, cellules C6, cellules COLO 320, cellules MCF-7
• Résultats positifs en IP: cellules MCF-7,
• Résultats positifs en IHC: tissu de tumeur ovarienne humain, tissu de cancer du côlon humain, tissu hépatique de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:8000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 6 publications below
• IHC: See 4 publications below
• IF: See 3 publications below
• IP: See 2 publications below
• RIP: See 1 publications below

Informations sur le produit

10770-1-AP cible MSI2 dans les applications de WB, IHC, IF, IP, RIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: MSI2 Protéine recombinante Ag1213
• Nom complet: musashi homolog 2 (Drosophila)
• Masse moléculaire calculée: 35 kDa
• Poids moléculaire observé: 37-40 kDa
• Numéro d’acquisition GenBank: BC001526
• Symbole du gène: MSI2
• Identification du gène (NCBI): 124540
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

MSI2 is an RNA-binding protein that regulates the expression of target mRNAs at the translation level. It binds to RNA via 2 ribonucleoprotein-type RNA recognition motifs in their N-termini, to consensus motifs in mRNAs to inhibit transcription. It may play a role in the proliferation and maintenance of stem cells in the central nervous system. MSI2 can regulate normal hematopoiesis and promote aggressive myeloid leukemia

Publications

Species	Application	Title
human	WB,IF	Science; Neurodevelopmental protein Musashi-1 interacts with the Zika genome and promotes viral replication.; Authors - Pavithra L Chavali
human	WB	EMBO J; Suppression of miR-199a maturation by HuR is crucial for hypoxia-induced glycolytic switch in hepatocellular carcinoma.; Authors - Ling-Fei Zhang
human	WB	Int J Oncol; Effects of three-dimensional collagen scaffolds on the expression profiles and biological functions of glioma cells.; Authors - Wei Jia
human	IHC	Mod Pathol; Progenitor stem cell marker expression by pulmonary carcinomas.; Authors - Moreira Andre L AL
human	WB, IP, IF	Front Mol Biosci; RNA-Binding Protein MSI2 Binds to miR-301a-3p and Facilitates Its Distribution in Mitochondria of Endothelial Cells.; Authors - Qian Qian Guo
human	IHC	Exp Cell Res; Msi2 plays a carcinogenic role in esophageal squamous cell carcinoma via regulation of the Wnt/β-catenin and Hedgehog signaling pathways.; Authors - Zhiwei Li

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